Original Article

JBIC Journal of Biological Inorganic Chemistry

, Volume 7, Issue 6, pp 640-645

First online:

Hard X-ray microprobe studies of chromium(VI)-treated V79 Chinese hamster lung cells: intracellular mapping of the biotransformation products of a chromium carcinogen

  • Carolyn T. DillonAffiliated withCentre for Heavy Metals Research, School of Chemistry, University of Sydney, NSW 2006, Australia
  • , Peter A. LayAffiliated withCentre for Heavy Metals Research, School of Chemistry, University of Sydney, NSW 2006, Australia
  • , Brendan J. KennedyAffiliated withCentre for Heavy Metals Research, School of Chemistry, University of Sydney, NSW 2006, Australia
  • , Anton P. StampflAffiliated withPhysics Division, Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW 2234, Australia
  • , Zhonghou CaiAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA
  • , Peter IlinskiAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA
  • , William RodriguesAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA
  • , Daniel G. LegniniAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA
  • , Barry LaiAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA
    • , Jörg MaserAffiliated withExperimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439, USA

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Abstract.

The uptake of carcinogenic and mutagenic Cr compounds and the intracellular distribution of their biotransformation products in V79 Chinese hamster lung cells were studied by synchrotron-radiation-induced X-ray emission (SRIXE). SRIXE analysis was performed on whole cells that had been treated with either Cr(III) or Cr(V) 1,10-phenanthroline complexes, or Cr(VI). The high spatial resolution (0.3 µm) and elemental sensitivity (~10–15 g Cr/cell) of the technique provided detailed maps of Cr and other cellular elements in thin sections prepared from Cr(VI)-treated cells. The Cr carcinogen concentrated in P-rich regions corresponding to the nucleus, as well as other areas of the cell that are likely to correspond to organelles. This is the first study that has enabled the determination of the localization of the biotransformation products of Cr(VI) carcinogens in a target lung cell. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-002-0343-5.

Chromium Cancer Synchrotron-radiation-induced X-ray emission Mapping Mammalian cells