Journal of Bone and Mineral Metabolism

, Volume 28, Issue 5, pp 503–509

Beneficial effects of vitamin E isomer supplementation on static and dynamic bone histomorphometry parameters in normal male rats

  • Muhammad Zulfadli Mehat
  • Ahmad Nazrun Shuid
  • Norazlina Mohamed
  • Norliza Muhammad
  • Ima Nirwana Soelaiman
Original Article

DOI: 10.1007/s00774-010-0159-2

Cite this article as:
Mehat, M.Z., Shuid, A.N., Mohamed, N. et al. J Bone Miner Metab (2010) 28: 503. doi:10.1007/s00774-010-0159-2

Abstract

Bone is a specialized connective tissue that functions as the load-bearing structure of the body. Free radicals may affect bone remodeling by regulating osteoclast activity in either the physiological or pathological condition. Vitamin E, a lipid-soluble antioxidant, has been demonstrated to offer protection against osteoporosis and to improve the bone material and structure of animal models. The aim of this study was to observe and compare the effects of alpha-tocopherol (α-tocopherol), delta-tocotrienol (δ-tocotrienol), and gamma-tocotrienol (γ-tocotrienol) on the static and dynamic bone histomorphometric parameters in normal male rats. Thirty-two normal Sprague–Dawley male rats aged 3 months and weighing 200–250 g were randomly divided into four groups. The control group was supplemented with oral gavages of olive oil (vehicle), whereas the α-tocopherol, δ-tocotrienol, and γ-tocotrienol groups were given oral gavages of 60 mg/kg α-tocopherol, δ-tocotrienol, and γ-tocotrienol, respectively. The rats were injected twice with calcein to fluorochrome-label the bones. After 4 months of treatment, the rats were killed, and the left femurs were dissected out and prepared for bone histomorphometry. Both the static and dynamic parameters of the vitamin E-treated groups were better than those of the normal control group. Among the vitamin E-treated groups, the tocotrienol groups showed better histomorphometry results compared to the α-tocopherol group, with the γ-tocotrienol group demonstrating the best effects on both sets of parameters. We concluded that vitamin E can promote bone formation in normal rats, with γ-tocotrienol being the most potent form of vitamin E.

Keywords

BoneVitamin EBone histomorphometryTocotrienolTocopherol

Copyright information

© The Japanese Society for Bone and Mineral Research and Springer 2010

Authors and Affiliations

  • Muhammad Zulfadli Mehat
    • 1
  • Ahmad Nazrun Shuid
    • 2
  • Norazlina Mohamed
    • 2
  • Norliza Muhammad
    • 2
  • Ima Nirwana Soelaiman
    • 2
  1. 1.Division of Pharmacology, Department of Human Anatomy, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of PharmacologyUniversiti Kebangsaan Malaysia Medical CentreKuala LumpurMalaysia