Amino Acids

, Volume 42, Issue 5, pp 2009–2017

Characterization of MRNP34, a novel methionine-rich nacre protein from the pearl oysters

  • Benjamin Marie
  • Caroline Joubert
  • Corinne Belliard
  • Alexandre Tayale
  • Isabelle Zanella-Cléon
  • Frédéric Marin
  • Yannick Gueguen
  • Caroline Montagnani
Original Article

DOI: 10.1007/s00726-011-0932-0

Cite this article as:
Marie, B., Joubert, C., Belliard, C. et al. Amino Acids (2012) 42: 2009. doi:10.1007/s00726-011-0932-0

Abstract

Nacre of the Pinctada pearl oyster shells is composed of 98% CaCO3 and 2% organic matrix. The relationship between the organic matrix and the mechanism of nacre formation currently constitutes the main focus regarding the biomineralization process. In this study, we isolated a new nacre matrix protein in P. margaritifera and P. maxima, we called Pmarg- and Pmax-MRNP34 (methionine-rich nacre protein). MRNP34 is a secreted hydrophobic protein, which is remarkably rich in methionine, and which is specifically localised in mineralizing the epithelium cells of the mantle and in the nacre matrix. The structure of this protein is drastically different from those of the other nacre proteins already described. This unusual methionine-rich protein is a new member in the growing list of low complexity domain containing proteins that are associated with biocalcifications. These observations offer new insights to the molecular mechanisms of biomineralization.

Keywords

Matrix protein Methionine-rich Biomineralization Mollusc Nacre Calcifying mantle 

Abbreviations

AA

Amino-acid

AIM

Acid-insoluble matrix

ASM

Acid-soluble matrix

EST

Expressed sequence tag

ISH

In situ hybridization

MRNP

Methionine-rich nacre protein

MS

Mass spectrometry

RLCD

Repeated low complexity domain

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Benjamin Marie
    • 1
    • 2
    • 3
  • Caroline Joubert
    • 1
  • Corinne Belliard
    • 1
  • Alexandre Tayale
    • 1
  • Isabelle Zanella-Cléon
    • 4
  • Frédéric Marin
    • 2
  • Yannick Gueguen
    • 1
  • Caroline Montagnani
    • 1
    • 5
  1. 1.Ifremer, LBQPCentre Océanologique du PacifiqueTaravaoFrench Polynesia
  2. 2.UMR 5561, CNRS, BiogéosciencesUniversité de BourgogneDijonFrance
  3. 3.UMR 7245, CNRS, MCAMMuséum National d’Histoire NaturelleParisFrance
  4. 4.UMR 5086, CNRS, IFR 128 BioSciences, IBCPUniversité de Lyon 1LyonFrance
  5. 5.UMR 5119, CNRS, Ifremer, IRD, Ecologie des Systèmes Marins CôtiersUniversité Montpellier IIMontpellierFrance

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