Amino Acids

, Volume 40, Issue 1, pp 113–122

Peptidomic analysis of the skin secretions of the frog Pachymedusa dacnicolor

Authors

  • Erika P. Meneses
    • Unidad de Proteómica-Instituto de BiotecnologíaUniversidad Nacional Autónoma de México (UNAM)
  • Oscar Villa-Hernández
    • Unidad de Proteómica-Instituto de BiotecnologíaUniversidad Nacional Autónoma de México (UNAM)
  • Lorena Hernández-Orihuela
    • Unidad de Proteómica-Instituto de BiotecnologíaUniversidad Nacional Autónoma de México (UNAM)
  • Ruben Castro-Franco
    • Lab. HerpetologíaCentro de Investigaciones Biológicas, UAEM
  • Victoria Pando
    • Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública
  • Manuel B. Aguilar
    • Laboratorio de Neurofarmacología Marina, Departamento de Neurobiologia Celular y MolecularInstituto de Neurobiología, UNAM
    • Unidad de Proteómica-Instituto de BiotecnologíaUniversidad Nacional Autónoma de México (UNAM)
Original article

DOI: 10.1007/s00726-010-0564-9

Cite this article as:
Meneses, E.P., Villa-Hernández, O., Hernández-Orihuela, L. et al. Amino Acids (2011) 40: 113. doi:10.1007/s00726-010-0564-9

Abstract

High-resolution mass spectrometry-based peptidomics has been used to characterize several components in electro-stimulated skin secretions of the endemic Mexican frog Pachymedusa dacnicolor. Peptide mass screening performed in an Orbitrap-XL mass spectrometer showed that P. dacnicolor skin secretions possess 194 different components with molecular masses ranging mainly from 500 to 6,000 Da. Dozens of molecules were partially sequenced including two novel protease inhibitors. Additionally, one posttranslationally modified bradykinin and two novel dermaseptin-like antimicrobial peptides were fully sequenced. The novel peptide named here DMS-DA5 was fully characterized and showed potent antibacterial activity against various bacteria such as Escherichia coli, Bacillus subtilis, Salmonella enterica serovar typhimurium, and Pseudomonas aeruginosa with minimal inhibitory concentrations from 3.10 to 25.0 μM.

Keywords

Frog skinAntibacterial peptidesPeptidomicsMass spectrometry

Copyright information

© Springer-Verlag 2010