Amino Acids

, Volume 38, Issue 1, pp 257–262

Transglutaminase-dependent antiproliferative and differentiative properties of nimesulide on B16-F10 mouse melanoma cells

  • Angelo Gismondi
  • Alessandro Lentini
  • Claudio Tabolacci
  • Bruno Provenzano
  • Simone Beninati
Original Article

DOI: 10.1007/s00726-009-0244-9

Cite this article as:
Gismondi, A., Lentini, A., Tabolacci, C. et al. Amino Acids (2010) 38: 257. doi:10.1007/s00726-009-0244-9

Abstract

The aim of this study was to collect evidences on the role of transglutaminase (TG, E.C.2.3.2.13) in the antineoplastic properties exerted by nimesulide (NMS), a non-steroidal anti-inflammatory drug, on murine B16-F10 melanoma cells. Treatment of melanoma cells with nimesulide produces a considerable reduction of cell proliferation, paralleled by a remarkable decrease of the intracellular concentration of polyamines spermidine and spermine. NMS treatment induces cancer cell differentiation, likely through the observed enhancement of TG and tyrosinase activities and increase of melanin production, well known markers of melanocyte differentiation. The overall results highlight the possibility that nimesulide acts as antineoplastic agent likely through the induction of intracellular TG activity.

Keywords

Nimesulide Polyamines Transglutaminase Differentiation Tyrosinase Melanin 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Angelo Gismondi
    • 1
  • Alessandro Lentini
    • 1
  • Claudio Tabolacci
    • 1
  • Bruno Provenzano
    • 1
  • Simone Beninati
    • 1
  1. 1.Department of BiologyUniversity “Tor Vergata”RomeItaly

Personalised recommendations