Amino Acids

, Volume 38, Issue 1, pp 65–73

Metabonomics and population studies: age-related amino acids excretion and inferring networks through the study of urine samples in two Italian isolated populations

  • Pio D’Adamo
  • Sheila Ulivi
  • Amerigo Beneduci
  • Gabriele Pontoni
  • Giovambattista Capasso
  • Carmela Lanzara
  • Gilberto Andrighetto
  • Uros Hladnik
  • Virginia Nunes
  • Manuel Palacin
  • Paolo Gasparini
Original Article

DOI: 10.1007/s00726-008-0205-8

Cite this article as:
D’Adamo, P., Ulivi, S., Beneduci, A. et al. Amino Acids (2010) 38: 65. doi:10.1007/s00726-008-0205-8

Abstract

The study of two different Italian isolated populations was combined with a metabonomic approach to better understand tubular handling of amino acids. Levels of amino acids and metabolites have been analyzed by Nucleic Magnetic Resonance and expressed as ratio vs urinary creatinine concentration (mmol/mol). For most of the amino acids there is an age-related U shape pattern of excretion, with the peaks during childhood and old age, and a significant reduction in the adult age. Hierarchical cluster analysis has clearly identified three groups clustering the same amino acids: His, Thr and Ala (group one); Gly and Phe (group two) and a third larger one. Results have been further confirmed by factor and regression analysis, and used to confirm and, in some cases, infer new amino acids networks. As a matter of facts, the identification of strong evidences for clustering of urine excretion of several neutral amino acids suggests the predominant impact of relevant and common transporters

Keywords

Amino acids Tubular handling Metabonomics Population studies 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Pio D’Adamo
    • 1
  • Sheila Ulivi
    • 1
  • Amerigo Beneduci
    • 2
  • Gabriele Pontoni
    • 3
  • Giovambattista Capasso
    • 4
  • Carmela Lanzara
    • 1
    • 8
  • Gilberto Andrighetto
    • 5
  • Uros Hladnik
    • 5
  • Virginia Nunes
    • 6
  • Manuel Palacin
    • 7
  • Paolo Gasparini
    • 1
    • 8
    • 9
  1. 1.Medical Genetics, Department of Laboratory MedicineInstitute for Maternal and Child Health IRCCS-Burlo GarofoloTriesteItaly
  2. 2.Department of ChemistryUniversity of CalabriaArcavacata di RendeItaly
  3. 3.Department of Biochemistry and Biophysics “Francesco Cedrangolo”Second University of NaplesNaplesItaly
  4. 4.Department of Internal Medicine, Section of NephrologySecond University of NaplesNaplesItaly
  5. 5.Medical GeneticsM.Baschirotto Institute for Rare Diseases. B.I.R.D. FoundationVicenzaItaly
  6. 6.CGMM-IDIBELL, Secció de genètica, Ciències Fisiològiques II, UB and CIBERER U730BarcelonaSpain
  7. 7.Institute for Research in Biomedicine, Barcelona Science Park, Department of Biochemistry and Molecular Biology, Faculty of BiologyUniversity of Barcelona, and CIBERERBarcelonaSpain
  8. 8.Medical Genetics, Department of Reproductive Sciences and DevelopmentUniversity of TriesteTriesteItaly
  9. 9.Medical Genetics, Department of Reproductive Sciences and DevelopmentInstitute for Maternal and Child Health IRCCS-Burlo Garofolo and University of TriesteTriesteItaly