Amino Acids

, Volume 37, Issue 4, pp 725–732

Source of oseltamivir resistance in avian influenza H5N1 virus with the H274Y mutation

  • Maturos Malaisree
  • Thanyada Rungrotmongkol
  • Nadtanet Nunthaboot
  • Ornjira Aruksakunwong
  • Pathumwadee Intharathep
  • Panita Decha
  • Pornthep Sompornpisut
  • Supot Hannongbua
Original Article

DOI: 10.1007/s00726-008-0201-z

Cite this article as:
Malaisree, M., Rungrotmongkol, T., Nunthaboot, N. et al. Amino Acids (2009) 37: 725. doi:10.1007/s00726-008-0201-z

Abstract

Molecular dynamics simulations were carried out for the mutant oseltamivir-NA complex, to provide detailed information on the oseltamivir-resistance resulting from the H274Y mutation in neuraminidase (NA) of avian influenza H5N1 viruses. In contrast with a previous proposal, the H274Y mutation does not prevent E276 and R224 from forming the hydrophobic pocket for the oseltamivir bulky group. Instead, reduction of the hydrophobicity and size of pocket in the area around an ethyl moiety at this bulky group were found to be the source of the oseltamivir-resistance. These changes were primarily due to the dramatic rotation of the hydrophilic –COO group of E276 toward the ethyl moiety. In addition, hydrogen-bonding interactions with N1 residues at the -NH3+ and -NHAc groups of oseltamivir were replaced by a water molecule. The calculated binding affinity of oseltamivir to NA was significantly reduced from −14.6 kcal mol−1 in the wild-type to −9.9 kcal mol−1 in the mutant-type.

Keywords

Oseltamivir resistance H274Y mutation Neuraminidase H5N1 

Supplementary material

726_2008_201_MOESM1_ESM.pdf (406 kb)
Supplementary table and figures (PDF 405 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Maturos Malaisree
    • 1
  • Thanyada Rungrotmongkol
    • 1
  • Nadtanet Nunthaboot
    • 2
  • Ornjira Aruksakunwong
    • 3
  • Pathumwadee Intharathep
    • 1
  • Panita Decha
    • 1
  • Pornthep Sompornpisut
    • 1
  • Supot Hannongbua
    • 1
  1. 1.Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Department of Chemistry, Faculty of ScienceMahasarakham UniversityMahasarakhamThailand
  3. 3.Department of Chemistry, Faculty of ScienceRangsit UniversityPathumtaniThailand