Amino Acids

, 37:629

Changes in amino acid concentration in plasma and type I and type II fibres during resistance exercise and recovery in human subjects

Original Article

DOI: 10.1007/s00726-008-0182-y

Cite this article as:
Blomstrand, E. & Essén-Gustavsson, B. Amino Acids (2009) 37: 629. doi:10.1007/s00726-008-0182-y


Eight male subjects performed leg press exercise, 4 × 10 repetitions at 80% of their maximum. Venous blood samples were taken before, during exercise and repeatedly during 2 h of recovery. From four subjects, biopsies were taken from the vastus lateralis muscle prior to, immediately after and following one and 2 h of recovery. Samples were freeze-dried, individual muscle fibres were dissected out and identified as type I or type II. Resistance exercise led to pronounced reductions in the glutamate concentration in both type I (32%) and type II fibres (70%). Alanine concentration was elevated 60–75% in both fibre types and 29% in plasma. Glutamine concentration remained unchanged after exercise; although 2 h later the concentrations in both types of fibres were reduced 30–35%. Two hours after exercise, the plasma levels of glutamate and six of the essential amino acids, including the branched-chain amino acids were reduced 5–30%. The data suggest that glutamate acts as an important intermediate in muscle energy metabolism during resistance exercise, especially in type II fibres.


Amino acidsMuscle fibre typesResistance exercise

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Swedish School of Sport and Health SciencesStockholmSweden
  2. 2.Department of Physiology and PharmacologyKarolinska InstitutetStockholmSweden
  3. 3.Department of Clinical SciencesSwedish University of Agricultural SciencesUppsalaSweden