Amino Acids

, Volume 36, Issue 4, pp 739–746

TIG3: a regulator of type I transglutaminase activity in epidermis

Authors

    • Department of Biochemistry and Molecular BiologyUniversity of Maryland School of Medicine
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
  • Michael T. Sturniolo
    • Department of Biochemistry and Molecular BiologyUniversity of Maryland School of Medicine
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
  • Ralph Jans
    • Department of Biochemistry and Molecular BiologyUniversity of Maryland School of Medicine
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
  • Catherine A. Kraft
    • Department of Biochemistry and Molecular BiologyUniversity of Maryland School of Medicine
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
  • Haibing Jiang
    • Department of Biochemistry and Molecular BiologyUniversity of Maryland School of Medicine
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
  • Ellen A. Rorke
    • Department of DermatologyUniversity of Maryland School of Medicine
    • Greenebaum Cancer CenterUniversity of Maryland School of Medicine
    • Department of Microbiology and ImmunologyUniversity of Maryland School of Medicine
Review Article

DOI: 10.1007/s00726-008-0123-9

Cite this article as:
Eckert, R.L., Sturniolo, M.T., Jans, R. et al. Amino Acids (2009) 36: 739. doi:10.1007/s00726-008-0123-9

Abstract

Keratinocytes undergo a process of terminal cell differentiation that results in the construction of a multilayered epithelium designed to produce a structure that functions to protect the body from dehydration, abrasion and infection. These protective properties are due to the production of a crosslinked layer of protein called the cornified envelope. Type I transglutaminase (TG1), an enzyme that catalyzes the formation of ε-(γ-glutamyl)lysine bonds, is the key protein responsible for generation of the crosslinks. The mechanisms that lead to activation of transglutaminase during terminal differentiation are not well understood. We have identified a protein that interacts with TG1 and regulates its activity. This protein, tazarotene-induced gene 3 (TIG3), is expressed in the differentiated layers of the epidermis and its expression is associated with transglutaminase activation and cornified envelope formation. We describe a novel mechanism whereby TIG3 regulates TG1 activity.

Keywords

TransglutaminaseTIG3Keratinocyte differentiationCalciumRIG1H-rev 107 tumor suppressor

Abbreviations

TG1

Transglutaminase type 1

TIG3

Tazarotene-induced gene 3

FC

Fluorescein cadaverine

Copyright information

© Springer-Verlag 2008