Amino Acids

, Volume 35, Issue 1, pp 217–224

Attenuation by dietary taurine of dextran sulfate sodium-induced colitis in mice and of THP-1-induced damage to intestinal Caco-2 cell monolayers

Authors

  • Z. Zhao
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ScienceThe University of Tokyo
  • H. Satsu
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ScienceThe University of Tokyo
  • M. Fujisawa
    • Department of Veterinary Pharmacology, Graduate School of Agriculture and Life SciencesThe University of Tokyo
  • M. Hori
    • Department of Veterinary Pharmacology, Graduate School of Agriculture and Life SciencesThe University of Tokyo
  • Y. Ishimoto
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ScienceThe University of Tokyo
  • M. Totsuka
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ScienceThe University of Tokyo
  • A. Nambu
    • Division of Cell Biology, Center for Experimental Medicine, Institute of Medical ScienceThe University of Tokyo
  • S. Kakuta
    • Division of Cell Biology, Center for Experimental Medicine, Institute of Medical ScienceThe University of Tokyo
  • H. Ozaki
    • Department of Veterinary Pharmacology, Graduate School of Agriculture and Life SciencesThe University of Tokyo
  • M. Shimizu
    • Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ScienceThe University of Tokyo
Article

DOI: 10.1007/s00726-007-0562-8

Cite this article as:
Zhao, Z., Satsu, H., Fujisawa, M. et al. Amino Acids (2008) 35: 217. doi:10.1007/s00726-007-0562-8
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Summary.

The effects of dietary taurine on the experimental colitis induced by dextran sulfate sodium (DSS) in mice were evaluated. C57BL/6 female mice were given 3% DSS in drinking water for 5 d to induce acute colitis. Taurine at 2% was added to the drinking water 5 d before and during the DSS-treatment to investigate its preventive effect. Taurine supplementation significantly attenuated the weight decrease, diarrhea severity, colon shortening, and the increase in the colonic tissue myeloperoxidase activity induced by DSS. Taurine also significantly inhibited the increase in the expression of a pro-inflammatory chemokine, macrophage inflammatory protein 2 (MIP-2), but not of interleukin (IL)-1β or tumor necrosis factor (TNF)-α mRNA. Furthermore, taurine significantly protected the intestinal Caco-2 cell monolayers from the damage by macrophage-like THP-1 cells in an in vitro coculture system. These results suggest that taurine prevented DSS-induced colitis partly in association with (1) its inhibitory effects on the secretion of MIP-2 from the intestinal epithelial cells and on the infiltration of such inflammatory cells as neutrophils and (2) its cytoprotective functions on the epithelial barrier from the direct toxicity of DSS and from the inflammatory cell-induced injury.

Keywords: Taurine – IBD – DSS – Caco-2 – Colitis

Abbreviations:

CD

Crohn’s disease

DSS

dextran sulfate sodium

IBDs

inflammatory bowel diseases

IL

interleukine

LDH

lactate dehydrogenase

MIP-2

macrophage inflammatory protein 2

MPO

myeloperoxidase

NF-κB

nuclear factor-κB

TNBS

trinitrobenzene sulfonic acid

TNF-α

tumor necrosis factor

UC

ulcerative colitis

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© Springer-Verlag 2007