Amino Acids

, Volume 32, Issue 4, pp 543–551

Sulfenic acid in human serum albumin

  • S. Carballal
  • B. Alvarez
  • L. Turell
  • H. Botti
  • B. A. Freeman
  • R. Radi
Review Article

DOI: 10.1007/s00726-006-0430-y

Cite this article as:
Carballal, S., Alvarez, B., Turell, L. et al. Amino Acids (2007) 32: 543. doi:10.1007/s00726-006-0430-y

Summary.

Sulfenic acid (RSOH) is a central intermediate in both the reversible and irreversible redox modulation by reactive species of an increasing number of proteins involved in signal transduction and enzymatic pathways. In this paper we focus on human serum albumin (HSA), the most abundant plasma protein, proposed to serve antioxidant functions in the vascular compartment. Sulfenic acid in HSA has been previously detected using different methods after oxidation of its single free thiol Cys34 through one- or two-electron mechanisms. Since recent evidence suggests that sulfenic acid in HSA is stabilized within the protein environment, this derivative represents an appropriate model to examine protein sulfenic acid biochemistry, structure and reactivity. Sulfenic acid in HSA could be involved in mixed disufide formation, supporting a role of HSA-Cys34 as an important redox regulator in extracellular compartments.

Keywords: Thiol – Human serum albumin – Sulfenic acid – Peroxynitrite – Hydrogen peroxide – Free radicals

Abbreviations:

HSA

human serum albumin

HSA-SH

the thiol of HSA

BSA

bovine serum albumin

SH/HSA

amount of thiol per albumin molecule

RSOH

sulfenic acid

NBD-Cl

7-chloro-4-nitrobenzo-2-oxa-1,3-diazol

DTNB

5,5′-dithio-bis(2-nitrobenzoic acid)

dimedone

5,5-dimethyl-1,3-cyclohexanedione

GSH

glutathione

GSSG

glutathione disulfide

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • S. Carballal
    • 1
    • 2
    • 3
  • B. Alvarez
    • 1
    • 3
  • L. Turell
    • 1
    • 3
  • H. Botti
    • 2
    • 3
  • B. A. Freeman
    • 4
  • R. Radi
    • 2
    • 3
  1. 1.Laboratorio de Enzimología, Facultad de CienciasUniversidad de la RepúblicaMontevideoUruguay
  2. 2.Departamento de Bioquímica, Facultad de MedicinaUniversidad de la RepúblicaMontevideoUruguay
  3. 3.Center for Free Radical and Biomedical ResearchUniversidad de la RepúblicaMontevideoUruguay
  4. 4.Department of PharmacologyUniversity of Pittsburgh Medical CenterPittsburghUSA