Amino Acids

, Volume 33, Issue 1, pp 51–56

Detecting oxidative post-translational modifications in proteins

  • E. Gianazza
  • J. Crawford
  • I. Miller
Mini-Review Article

DOI: 10.1007/s00726-006-0410-2

Cite this article as:
Gianazza, E., Crawford, J. & Miller, I. Amino Acids (2007) 33: 51. doi:10.1007/s00726-006-0410-2

Summary.

Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys, Tyr, Met, Trp, Lys).

Keywords: Oxidative post-translational modifications – Proteomics – Electrophoresis – Carbonylation – Glutathionylation

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • E. Gianazza
    • 1
  • J. Crawford
    • 2
  • I. Miller
    • 3
  1. 1.Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze FarmacologicheUniversità degli Studi di MilanoMilanoItaly
  2. 2.Nonlinear Dynamics Ltd.Newcastle-upon-TyneU.K.
  3. 3.Department für Naturwissenschaften, Institut für Medizinische ChemieVeterinärmedizinische Universität WienWienAustria