Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength
First Online: 20 September 2006 Received: 13 April 2006 Accepted: 17 July 2006 DOI:
Cite this article as: Willoughby, D., Stout, J. & Wilborn, C. Amino Acids (2007) 32: 467. doi:10.1007/s00726-006-0398-7 Summary.
This study examined 10 wks of resistance training and the ingestion of supplemental protein and amino acids on muscle performance and markers of muscle anabolism. Nineteen untrained males were randomly assigned to supplement groups containing either 20 g protein (14 g whey and casein protein, 6 g free amino acids) or 20 g dextrose placebo ingested 1 h before and after exercise for a total of 40 g/d. Participants exercised 4 times/wk using 3 sets of 6–8 repetitions at 85–90% of the one repetition maximum. Data were analyzed with two-way ANOVA (
p < 0.05). The protein supplement resulted in greater increases in total body mass, fat-free mass, thigh mass, muscle strength, serum IGF-1, IGF-1 mRNA, MHC I and IIa expression, and myofibrillar protein. Ten-wks of resistance training with 20 g protein and amino acids ingested 1 h before and after exercise is more effective than carbohydrate placebo in up-regulating markers of muscle protein synthesis and anabolism along with subsequent improvements in muscle performance. Keywords: Resistance training – Insulin – Myosin heavy chain – IGF-1 – Amino acids References Adams, G 1998 Role of insulin-like growth factor-I in the regulation of skeletal muscle adaptation to increased loading Exerc Sport Sci Rev 26 31 60 PubMed CrossRef Google Scholar American College of Sports Medicine 2000Guidelines for exercise testing and prescription6 Lippincott Williams and Wilkins New York 24 26 Google Scholar Andersen, P, Saltin, B 1985 Maximal perfusion of skeletal muscle in man J Physiol 366 233 249 PubMed Google Scholar Andersen, L, Tufekovic, G, Zebis, M, Crameri, R, Verlaan, G, Khaer, M, Suetta, C, Magnusson, P, Aagaard, P 2005 The effect of resistance training combined with timed ingestion of protein on muscle fiber size and muscle strength Metabolism 54 151 156 PubMed CrossRef Google Scholar Baechle, T, Earle, R, Wathen, D 2000 Resistance training Baechle, T Earle, R eds. Essentials of strength training and conditioning2 Human Kinetic Champaign, IL 420 421 Google Scholar Ballard, T, Clapper, J, Specker, B, Binkley, T, Vukovich, M 2005 Effect of protein supplementation during a 6-mo strength and conditioning program on insulin-like growth factor I and markers of bone turnover in young adults Am J Clin Nutr 81 1442 1448 PubMed Google Scholar Bamman, M, Shipp, J, Jiang, J, Gower, B, Hunter, G, Goodman, A, McLafferty, C, Urban, R 2001 Mechanical load increase muscle IGF-1 and androgen receptor mRNA concentrations in humans Am J Physiol Endocrinol Metab 280 E383 E390 PubMed Google Scholar Biolo, G, Tipton, K, Klein, S, Wolfe, R 1997 An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein Am J Physiol 273 E122 E129 PubMed Google Scholar Bird, S, Tarpenning, K, Marino, K 2006 Independent and combined effects of liquid carbohydrate/essential amino acid ingestion on hormonal and muscular adaptations following resistance training in untrained men Eur J Appl Physiol 3 1 14 Google Scholar Bolster, D, Jefferson, L, Kimball, S 2004 Regulation of protein synthesis associated with skeletal muscle hypertrophy by insulin-, amino acid-, and exercise-induced signaling Proc Nutr Soc 63 351 356 PubMed CrossRef Google Scholar Borsheim, E, Aarsland, A, Wolfe, R 2004a Effect of an amino acid, protein, and carbohydrate mixture on net muscle protein balance after resistance exercise Int J Sport Nutr Exerc Metab 14 255 271 Google Scholar Borsheim, E, Cree, M, Tipton, K, Elliott, T, Aarsland, A, Wolfe, R 2004b Effect of carbohydrate intake of net protein synthesis during recovery from resistance exercise J Appl Physiol 96 674 678 CrossRef Google Scholar Bowtell, J, Leese, G, Smith, K, Watt, P, Nevill, A, Rooyackers, O, Wagenmakers, A, Rennie, M 2000 Effect of oral glucose on leucine turnover in human subjects at rest and during exercise at two levels of dietary protein J Physiol 525 271 281 PubMed CrossRef Google Scholar Bradford, M 1976 A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding Anal Biochem 72 248 254 PubMed CrossRef Google Scholar Carroll, C, Fluckey, J, Williams, R, Sullivan, D, Trappe, T 2005 Human solues and vastus lateralis muscle protein metabolism with an amino acid infusion Am J Physiol Endocrinol Metab 288 E479 E485 PubMed CrossRef Google Scholar Chomczynski, P, Sacchi, N 1987 Single-step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction Anal Biochem 162 156 159 PubMed CrossRef Google Scholar
Current Protocols in Molecular Biology (1999) Short protocols in molecular biology. Wiley, New York, pp A3: 10–11
Dangin, M, Boirie, Y, Garcia-Rodenas, C, Gachon, P, Fauquant, J, Gallier, P, Ballevre, O, Beaufrere, B 2001 The digestion rate of protein is an independent regulating factor of postprandial protein retention Am J Physiol Endocrinol Metab 280 E340 E348 PubMed Google Scholar Hameed, M, Lange, K, Andersen, J, Schjerling, P, Kjaer, M, Harridge, D, Goldspink, G 2003 The effect of recombinant growth hormone and resistance training on IGF-1 mRNA expression in the muscles of elderly men J Physiol 555 231 240 PubMed CrossRef Google Scholar Karlsson, H, Nilsson, P, Nilsson, J, Chibalin, A, Zierath, J, Blomstrand, E 2004 Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise Am J Physiol Endocrinol Metab 287 E1 E7 PubMed CrossRef Google Scholar Koopman, R, Wagenmakers, A, Manders, R, Zorenc, A, Senden, J, Gorselink, M, Keizer, H, van Loon, L 2005 Combined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects Am J Physiol Endocrinol Metab 288 E645 E653 PubMed CrossRef Google Scholar Mahoney, D, Carey, K, Fu, M, Cameron-Smith, D, Parise, G, Tarnopolsky, M 2004 Real-time RT-PCR analysis of housekeeping genes in human skeletal muscle following acute exercise Physiol Genomics 8 226 231 Google Scholar Phillips, S, Tipton, K, Aarsland, A, Wolf, S, Wolfe, R 1997 Mixed muscle protein synthesis and breakdown after resistance exercise in humans Am J Physiol 273 E99 E107 PubMed Google Scholar Psilander, N, Damsgaard, R, Pilegaard, H 2003 Resistance exercise alters MRG and IFG-1 mRNA content in human skeletal muscle J Appl Physiol 95 1038 1044 PubMed Google Scholar Rasmussen, B, Tipton, K, Miller, S, Wolf, S, Wolfe, R 2000 An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise J Appl Physiol 88 386 392 PubMed Google Scholar Roy, D, Tarnopolsky, M, MacDougall, J, Fowles, J, Yarasheski, K 1997 Effect of glucose supplement timing on protein metabolism after resistance training J Appl Physiol 82 1882 1888 PubMed CrossRef Google Scholar Roy, D, Fowles, J, Hill, R, Tarnopolsky, M 2000 Macronutrient intake and whole body protein metabolism following resistance exercise Med Sci Sports Exerc 32 1412 1418 PubMed CrossRef Google Scholar Sartorelli, V, Fulco, M 2004 Molecular and cellular determinants of skeletal muscle atrophy and hypertrophy Sci STKE 244 re11 CrossRef Google Scholar Tipton, K, Elliott, T, Cree, M, Wolf, S, Sanford, A, Wolfe, R 2004 Ingestion of casein and whey proteins result in muscle anabolism after resistance exercise Med Sci Sports Exerc 36 2073 2081 PubMed CrossRef Google Scholar Walberg-Rankin, J, Goldman, L, Puglisi, M, Nickols-Richardson, S, Earthman, C, Gwazdauskas, F 2004 Effect of post-exercise supplement consumption on adaptations to resistance training J Am Coll Nutr 23 322 330 Google Scholar Welle, S, Bhatt, K, Thornton, C 1999 Stimulation of myofibrillar synthesis by exercise is mediated by more efficient translation of mRNA J Appl Physiol 86 1220 1225 PubMed Google Scholar Willoughby, D 2004a Effects of an alleged myostatin-binding supplement and heavy resistance training on serum myostatin muscle strength and mass, and body composition Int J Sport Nutr Exerc Metab 14 461 472 Google Scholar Willoughby, D 2004b Effects of heavy resistance training on myostatin mRNA and protein expression Med Sci Sports Exerc 36 574 582 CrossRef Google Scholar Willoughby, D, Nelson, M 2002 Myosin heavy-chain mRNA expression after a single session of heavy-resistance exercise Med Sci Sports Exerc 34 1262 1269 PubMed CrossRef Google Scholar Willoughby, D, Rosene, J 2001 Effects of oral creatine and resistance training on myosin heavy chain expression Med Sci Sports Exerc 33 1674 1681 PubMed CrossRef Google Scholar Yang, S, Alnaqeeb, M, Simpson, H, Goldspink, J 1996 Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch J Muscle Res Cell Motil 17 487 495 PubMed CrossRef Google Scholar