Amino Acids

, Volume 28, Issue 3, pp 279–290

Expression profiling using human tissues in combination with RNA amplification and microarray analysis: assessment of Langerhans cell histiocytosis

  • K. L. McClain
  • Y.-H. Cai
  • J. Hicks
  • L. E. Peterson
  • X.-T. Yan
  • S. Che
  • S. D. Ginsberg
Minireview Article

DOI: 10.1007/s00726-005-0177-x

Cite this article as:
McClain, K., Cai, Y., Hicks, J. et al. Amino Acids (2005) 28: 279. doi:10.1007/s00726-005-0177-x

Summary.

Advances in molecular genetics have led to sequencing of the human genome, and expression data is becoming available for many diverse tissues throughout the body, allowing for exciting hypothesis testing of critical concepts such as development, differentiation, homeostasis, and ultimately, disease pathogenesis. At present, an optimal methodology to assess gene expression is to evaluate single cells, either identified physiologically in living preparations, or by immunocytochemical or histochemical procedures in fixed cells in vitro or in vivo. Unfortunately, the quantity of RNA harvested from a single cell is not sufficient for standard RNA extraction methods. Therefore, exponential polymerase-chain reaction (PCR) based analyses, and linear RNA amplification including amplified antisense (aRNA) RNA amplification and a newly developed terminal continuation (TC) RNA amplification methodology have been used in combination with microdissection procedures such as laser capture microdissection (LCM) to enable the use of microarray platforms within individual populations of cells obtained from a variety of human tissue sources such as biopsy-derived samples {including Langerhans cell histiocytosis (LCH)} as well as postmortem brain samples for high throughput expression profiling and related downstream genetic analyses.

Keywords: cDNA microarray – Postmortem human brain – Cancer genomics – Translational neuroscience – Molecular fingerprint

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • K. L. McClain
    • 1
  • Y.-H. Cai
    • 1
  • J. Hicks
    • 2
  • L. E. Peterson
    • 3
  • X.-T. Yan
    • 1
  • S. Che
    • 4
    • 5
  • S. D. Ginsberg
    • 4
    • 5
    • 6
  1. 1.Texas Children’s Cancer Center/Hematology Service, Baylor College of MedicineHoustonU.S.A.
  2. 2.Pediatric Pathology, Baylor College of MedicineHoustonU.S.A.
  3. 3.Department of MedicineBaylor College of MedicineHoustonU.S.A.
  4. 4.Center for Dementia Research, Nathan Kline InstituteOrangeburgU.S.A.
  5. 5.Department of PsychiatryNew York University School of MedicineOrangeburgU.S.A.
  6. 6.Department of Physiology & NeuroscienceNew York University School of MedicineOrangeburgU.S.A.