Amino Acids

, Volume 27, Issue 3, pp 269–275

Aberrant cytosolic acyl-CoA thioester hydrolase in hippocampus of patients with mesial temporal lobe epilepsy

Authors

  • J. W. Yang
    • Department of PediatricsMedical University of Vienna
  • T. Czech
    • Department of NeurosurgeryMedical University of Vienna
  • J. Yamada
    • Department of Clinical BiochemistryTokyo University of Pharmacy and Life Science
  • E. Csaszar
    • Mass Spectrometry Unit, University of Vienna
  • C. Baumgartner
    • Department of Neurology, Section of Clinical Epilepsy ResearchMedical University of Vienna
  • I. Slavc
    • Department of PediatricsMedical University of Vienna
  • G. Lubec
    • Department of PediatricsMedical University of Vienna
Article

DOI: 10.1007/s00726-004-0138-9

Cite this article as:
Yang, J., Czech, T., Yamada, J. et al. Amino Acids (2004) 27: 269. doi:10.1007/s00726-004-0138-9

Summary.

A series of enzyme alterations has been shown to be associated with several forms of epilepsy, in mesial temporal lobe epilepsy (MTLE), however, information is limited. It was therefore the aim of the study to determine brain enzyme protein expression using a proteomic screening approach. Hippocampi of controls and patients with drug-resistant MTLE were used for evaluation of protein expression. We applied two-dimensional electrophoresis (2-DE) with mass spectrometrical identification and immunoblotting. 2-DE revealed a remarkably decreased spot identified as cytosolic acyl-CoA thioester hydrolase (BACH; EC 3.1.2.2) in patients with MTLE. Western blotting showed absence of bands at 37 kDa in MTLEs using an antibody against mouse BACH and at 140 kDa in MTLEs using anti-rat BACH. This study demonstrates that BACHs were deranged in hippocampus of MTLE patients. This finding may well contribute to the understanding of the still elusive pathomechanisms involved in MTLE.

Keywords: Acyl-CoA thioester hydrolase – Epilepsy – Hippocampus – Proteomics

Copyright information

© Springer-Verlag/Wien 2004