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Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy

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Abstract

CHAPS is a zwitterionic surfactant derivative of bile salts, widely used in membrane protein isolation. While some studies regarding CHAPS self-aggregation suggest continuous increase in micelle size at increasing CHAPS concentration, other works point to the existence of two definite micelle types. In this work, stearic acid spin labels (5, 12, or 16-SASL) were added to CHAPS solutions to obtain information about the micellar structure using electron paramagnetic resonance spectroscopy. The spectra of 12-SASL were processed using Principal Factor Analysis, and at all concentrations they could be reproduced as linear combinations of only three fundamental spectra, the first one corresponding to free 12-SASL in aqueous solution. This fact suggests only two hydrophobic environments that host 12-SASL, assigned to primary and secondary CHAPS micelles. The relative populations of the label in each environment were obtained as a function of CHAPS concentration. Our results suggest barrel-shaped primary micelles with a minimum mean radius of 1.46 nm, and a critical micelle concentration cmcI = 4 mM. Secondary micelles are formed by aggregation of primary ones, with cmcII = 10 mM. They have several elongated hydrophobic pockets, with similar dimensions for all aggregate sizes. Our results contribute to the understanding of the mechanism of interaction of chain amphiphiles with CHAPS micelles.

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Acknowledgments

This work was supported by CONICET and Universidad Nacional del Litoral. AMG is a researcher of CONICET. PMR had a scholarship from CONICET during part of the execution of this work. We thank Dr RR Koropecki for kindly allowing us the use of his PFA programs, and providing useful advice, and Dr Raúl Urteaga for helpful discussions.

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Authors and Affiliations

  1. Departamento de Física, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral (UNL), Ciudad Universitaria, 3000, Santa Fe, Argentina

    Pablo M. Rodi, María D. Bocco Gianello & Ana M. Gennaro

  2. IFIS Litoral (CONICET-UNL), Güemes 3450, 3000, Santa Fe, Argentina

    Ana M. Gennaro

Authors
  1. Pablo M. Rodi
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  2. María D. Bocco Gianello
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  3. Ana M. Gennaro
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Correspondence to Ana M. Gennaro.

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Rodi, P.M., Bocco Gianello, M.D. & Gennaro, A.M. Insights About CHAPS Aggregation Obtained by Spin Label EPR Spectroscopy. Appl Magn Reson 45, 1319–1332 (2014). https://doi.org/10.1007/s00723-014-0604-9

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