Applied Magnetic Resonance

, Volume 43, Issue 3, pp 311–320

An ESR, Mass Spectrometry and Fluorescence Microscopy Approach to Study the Stearic Acid Derivatives Anchoring in Cells

  • A. Grammenos
  • M. Fillet
  • M. Collodoro
  • S. Lecart
  • C. Quoilin
  • M.-P. Fontaine-Aupart
  • M. Hoebeke
Article

DOI: 10.1007/s00723-012-0375-0

Cite this article as:
Grammenos, A., Fillet, M., Collodoro, M. et al. Appl Magn Reson (2012) 43: 311. doi:10.1007/s00723-012-0375-0

Abstract

Lateral phase separations in biological membranes are of great interest, making electron spin resonance (ESR) spectroscopy combined with spin labeling a nondestructive and sensitive technique for the study of lipid rafts. It is currently accepted that the spin probe is localized on the plasma membrane. However, no study confirms this hypothesis. Herein, we report, for the first time, an accurate multispectral method for the quantification of the lipid spin label presence in every subcellular fraction. Cells were incubated with the 5-DOXYL stearic acid derivative and then subfractionated. Results of our multimodal spectroscopy approach ubiquitously demonstrate that the ESR spin label presence only sets in the plasma membranes.

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • A. Grammenos
    • 1
  • M. Fillet
    • 2
  • M. Collodoro
    • 3
    • 4
  • S. Lecart
    • 5
  • C. Quoilin
    • 1
  • M.-P. Fontaine-Aupart
    • 6
  • M. Hoebeke
    • 1
  1. 1.Laboratory of Biomedical Spectroscopy, Department of Physics, B5University of LiègeLiègeBelgium
  2. 2.Laboratory of Analytical Pharmaceutical Chemistry, Department of PharmacyDrug Research Center, University of LiègeLiègeBelgium
  3. 3.Laboratory of Histology-Cytology, Departement of ChemistryUniversity of LiègeLiègeBelgium
  4. 4.Laboratory of Mass Spectrometry, GIGA-R and CART, Department of ChemistryUniversity of LiègeLiègeBelgium
  5. 5.Center of Biomedical Photonic of LUMAT FederationUniversity of Paris-SudOrsayFrance
  6. 6.Institut of Molecular Sciences of OrsayUniversity of Paris-SudOrsayFrance