Protoplasma

, Volume 219, Issue 1, pp 116–121

Spatio-temporal relationship between nuclear-envelope breakdown and preprophase band disappearance in cultured tobacco cells

  • R. Dixit
  • R. J. Cyr
Rapid communication

DOI: 10.1007/s007090200012

Cite this article as:
Dixit, R. & Cyr, R. Protoplasma (2002) 219: 116. doi:10.1007/s007090200012

Summary.

Cell division involves the coordinated progression of karyokinesis and cytokinesis, which is accomplished by communication between the nucleus and the cytoplasm. We have utilized green-fluorescent-protein technology to generate a line of tobacco 'Bright Yellow 2' (BY-2) cells labeled for both microtubules and the nuclear envelope. This cell line allowed us to use living cells to investigate the relationship between nuclear-envelope breakdown and preprophase band disappearance with high spatial and temporal resolution. Our observations demonstrate that nuclear-envelope breakdown always precedes preprophase band disappearance in BY-2 cells. In addition, the rate of preprophase band disappearance, and the attenuation of perinuclear microtubule fluorescence, correlates with the proximity of the nucleus to the preprophase band site. These results indicate the presence of communication between the nucleus and the preprophase band and suggest a causal relationship between nuclear-envelope breakdown and preprophase band dis-appearance.

Keywords: Preprophase band; Nuclear-envelope breakdown; Prometaphase; Green-fluorescent protein; Red-fluorescent protein. 

Copyright information

© Springer-Verlag Wien 2002

Authors and Affiliations

  • R. Dixit
    • 1
  • R. J. Cyr
    • 1
  1. 1.Department of Biology, Pennsylvania State University, University Park, PennsylvaniaUS