Protoplasma

, Volume 254, Issue 1, pp 271–283

Jasmonates are induced by the PAMP flg22 but not the cell death-inducing elicitor Harpin in Vitis rupestris

  • Xiaoli Chang
  • Mitsunori Seo
  • Yumiko Takebayashi
  • Yuji Kamiya
  • Michael Riemann
  • Peter Nick
Original Article

DOI: 10.1007/s00709-016-0941-7

Cite this article as:
Chang, X., Seo, M., Takebayashi, Y. et al. Protoplasma (2017) 254: 271. doi:10.1007/s00709-016-0941-7

Abstract

Plants employ two layers of defence that differ with respect to cell death: pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI). In our previous work, we have comparatively mapped the molecular events in a cell system derived from the wild American grape Vitis rupestris, where cell death-independent defence can be triggered by PAMP flg22, whereas the elicitor Harpin activates a cell death-related ETI-like response. Both defence responses overlapped with respect to early events, such as calcium influx, apoplastic alkalinisation, oxidative burst, mitogen-activated protein kinase (MAPK) signalling, activation of defence-related genes and accumulation of phytoalexins. However, timing and amplitude of early signals differed. In the current study, we address the role of jasmonates (JAs) as key signalling compounds in hypersensitive cell death. We find, in V. rupestris, that jasmonic acid and its bioactive conjugate jasmonoyl-isoleucine (JA-Ile) rapidly accumulate in response to flg22 but not in response to Harpin. However, Harpin can induce programmed cell death, whereas exogenous methyl jasmonate (MeJA) fails to do so, although both signals induce a similar response of defence genes. Also in a second cell line from V. vinifera cv. ‘Pinot Noir’, where Harpin cannot activate cell death and where flg22 fails to induce JA and JA-Ile, defence genes are activated in a similar manner. These findings indicate that the signal pathway culminating in cell death must act independently from the events culminating in the accumulation of toxic stilbenes.

Keywords

Defence signalling Effector-triggered immunity (ETI) Jasmonic acid (JA) PAMP-triggered immunity (PTI) Vitis 

Supplementary material

709_2016_941_MOESM1_ESM.doc (89 kb)
ESM 1(DOC 89 kb)

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Xiaoli Chang
    • 1
    • 3
  • Mitsunori Seo
    • 2
  • Yumiko Takebayashi
    • 2
  • Yuji Kamiya
    • 2
  • Michael Riemann
    • 3
  • Peter Nick
    • 3
  1. 1.Department of Plant Pathology, Agricultural CollegeSichuan Agricultural UniversityChengduPeople’s Republic of China
  2. 2.RIKEN Center for Sustainable Resource ScienceYokohamaJapan
  3. 3.Molecular Cell Biology, Botanical InstituteKarlsruhe Institute of TechnologyKarlsruheGermany