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Biocompatibility of different nanostructured TiO2 scaffolds and their potential for urologic applications

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Abstract

Despite great efforts in tissue engineering of the ureter, urinary bladder, and urethra, further research is needed in order to improve the patient’s quality of life and minimize the economic burden of different lower urinary tract disorders. The nanostructured titanium dioxide (TiO2) scaffolds have a wide range of clinical applications and are already widely used in orthopedic or dental medicine. The current study was conducted to synthesize TiO2 nanotubes by the anodization method and TiO2 nanowires and nanospheres by the chemical vapor deposition method. These scaffolds were characterized with scanning electron microscopy (SEM) and X-ray diffraction (XRD) methods. In order to test the urologic applicability of generated TiO2 scaffolds, we seeded the normal porcine urothelial (NPU) cells on TiO2 nanotubes, TiO2 nanowires, TiO2 nanospheres, and on the standard porous membrane. The viability and growth of the cells were monitored everyday, and after 3 weeks of culturing, the analysis with scanning electron microscope (SEM) was performed. Our results showed that the NPU cells were attached on all scaffolds; they were viable and formed a multilayered epithelium, i.e., urothelium. The apical plasma membrane of the majority of superficial NPU cells, grown on all three different TiO2 scaffolds and on the porous membrane, exhibited microvilli; thus, indicating that they were at a similar differentiation stage. The maximal caliper diameter measurements of superficial NPU cells revealed significant alterations, with the largest cells being observed on nanowires and the smallest ones on the porous membrane. Our findings indicate that different nanostructured TiO2 scaffolds, especially nanowires, have a great potential for tissue engineering and should be further investigated for various urologic applications.

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Acknowledgments

We express gratitude to Sanja Čabraja, Nada Pavlica Dubarič, Linda Štrus, and Sabina Železnik for their technical assistance. This study was supported by a grant from the Slovenian Research Agency ARRS P2-0232 and P3-0108.

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Correspondence to Aleš Iglič.

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The experiments were approved by the Veterinary Administration of the Slovenian Ministry of Agriculture and Forestry (permit no. 34401-1/2010/6) in compliance with the Animal Health Protection Act and Instructions for Granting Permits for Animal Experimentation for Scientific Purposes.

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The authors declare that they have no conflict of interest.

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Handling Editor: Christos D. Katsetos

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Imani, R., Pazoki, M., Zupančič, D. et al. Biocompatibility of different nanostructured TiO2 scaffolds and their potential for urologic applications. Protoplasma 253, 1439–1447 (2016). https://doi.org/10.1007/s00709-015-0896-0

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  • DOI: https://doi.org/10.1007/s00709-015-0896-0

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