Protoplasma

, Volume 250, Issue 4, pp 919–929

A non-surgical method for induction of lung cancer in Wistar rats using a combination of NNK and high dietary fats

  • Shilpa Bhatnagar
  • Naveen Chaudhary
  • Deepshikha Pande Katare
  • S. K. Jain
Original Article

DOI: 10.1007/s00709-012-0478-3

Cite this article as:
Bhatnagar, S., Chaudhary, N., Katare, D.P. et al. Protoplasma (2013) 250: 919. doi:10.1007/s00709-012-0478-3

Abstract

Lung cancer is one of the most common malignant neoplasms all over the world. Smoking and a number of constituents of tobacco are responsible for development of lung tumours; however, the deleterious effects of tobacco-derived carcinogen, nitrosamine 4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone (nicotine-derived nitrosamine ketone (NNK)) remain unmatched. We report the development of a novel rodent model by administering multiple doses of NNK to male Wistar rats and feeding them with high-fat and low-protein diet. Tumour cells in lungs were observed in approximately 98 % rats after 8 months of NNK treatment, as evident by histopathological analysis. This rodent model showed slow progression of lung tumours which has helped us to assess early indicators of oxidative damage in lungs by studying the levels of lipid peroxidation and antioxidant parameters. LPO was elevated by 46.94 %, SOD, CAT, GSH and GR activity was decreased by 48.67 %, 22.04 %, 21.46 % and 20.85 %, respectively in serum of NNK treated rats when compared with control. These findings suggest that increased oxidative stress can represent a risk factor for the development of chronic disease in early future. This new animal model is an attempt to greatly facilitate studies of the pathophysiology, biochemistry and therapy of lung cancer.

Keywords

NNK Histopathology Lung tumours Oxidative damage 

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Shilpa Bhatnagar
    • 1
  • Naveen Chaudhary
    • 1
  • Deepshikha Pande Katare
    • 2
  • S. K. Jain
    • 1
  1. 1.Department of BiotechnologyHamdard UniversityNew DelhiIndia
  2. 2.Amity Institute of BiotechnologyAmity UniversityNoidaIndia

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