Protoplasma

, Volume 250, Issue 1, pp 185–196

Effect of heat shock on ultrastructure and calcium distribution in Lavandula pinnata L. glandular trichomes

Original Article

DOI: 10.1007/s00709-012-0393-7

Cite this article as:
Huang, S.S., Kirchoff, B.K. & Liao, J.P. Protoplasma (2013) 250: 185. doi:10.1007/s00709-012-0393-7

Abstract

The effects of heat shock (HS) on the ultrastructure and calcium distribution of Lavandula pinnata secretory trichomes are examined using transmission electron microscopy and potassium antimonate precipitation. After 48-h HS at 40°C, plastids become distorted and lack stroma and osmiophilic deposits, the cristae of the mitochondria become indistinct, the endoplasmic reticulum acquires a chain-like appearance with ribosomes prominently attached to the lamellae, and the plasma and organelle membranes become distorted. Heat shock is associated with a decrease in calcium precipitates in the trichomes, while the number of precipitates increases in the mesophyll cells. Prolonged exposure to elevated calcium levels may be toxic to the mesophyll cells, while the lack of calcium in the glands cell may deprive them of the normal protective advantages of elevated calcium levels. The inequality in calcium distribution may result not only from uptake from the transpiration stream, but also from redistribution of calcium from the trichomes to the mesophyll cells.

Keywords

Lavandula pinnata LGlandular trichomesHeat shockCalciumAntimonate precipitatesUltrastructure

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Guangdong Research Institute of Traditional Chinese MedicineGuangzhouChina
  2. 2.Department of BiologyUniversity of North Carolina at GreensboroGreensboroUSA
  3. 3.Key Laboratory of Digital Botanical Garden in Guangdong, South China Botanical GardenChinese Academy of SciencesGuangzhouChina