Protoplasma

, Volume 227, Issue 2, pp 113–118

Chromosome endoreduplication as a factor of salt adaptation in Sorghum bicolor

Authors

  • M. Ceccarelli
    • Dipartimento di Biologia Cellulare e AmbientaleUniversità di Perugia
  • E. Santantonio
    • Dipartimento di Biologia Cellulare e AmbientaleUniversità di Perugia
  • F. Marmottini
    • Dipartimento di Ingegneria CivileUniversità di Perugia
  • G. N. Amzallag
    • Judea Center for Research and Development
    • Dipartimento di Biologia Cellulare e AmbientaleUniversità di Perugia
Article

DOI: 10.1007/s00709-005-0144-0

Cite this article as:
Ceccarelli, M., Santantonio, E., Marmottini, F. et al. Protoplasma (2006) 227: 113. doi:10.1007/s00709-005-0144-0

Summary.

Nuclear DNA amounts were measured by Feulgen cytophotometry in Sorghum bicolor cv. 610 plants early exposed to 150 mM NaCl, a treatment known to induce an increased tolerance to salinity in plants carrying this genotype. In salt-treated plants, the percentages of 8C, 16C, and 32C nuclei in roots in the primary state of growth were 21.9%, 13.3%, and 4.3%, respectively. By contrast, in nonsalinized plants, only 3.5% of the nuclei had an 8C content and no higher DNA contents were observed. The salt treatment induced chromosome endoreduplication during the differentiation of cells in the root cortex, where 41.2% of the cells displayed a DNA content higher than 4C (versus 1.3% in control plants). No enhancement of endopolyploidy was observed in cells of the root vascular cylinder or the leaves of the salt-treated plants. In another S. bicolor genotype (DK 34-Alabama), noncompetent for salt adaptation, the same NaCl treatment did not induce chromosome endoreduplication in root cortex cells. Endopolyploidy may be considered as a part of the adaptive response of S. bicolor competent genotypes to salinity.

Keywords: Chromosome endoreduplication; Feulgen cytophotometry; Root cortex cell; Salt adaptation; Sorghum bicolor.
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© Springer-Verlag 2006