Monatshefte für Chemie / Chemical Monthly

, Volume 136, Issue 3, pp 367–395

Fluorescence Detected Exciton Coupled Circular Dichroism: Development of New Fluorescent Reporter Groups for Structural Studies

  • Katsunori Tanaka
  • Gennaro Pescitelli
  • Koji Nakanishi
  • Nina Berova
Article

DOI: 10.1007/s00706-004-0276-5

Cite this article as:
Tanaka, K., Pescitelli, G., Nakanishi, K. et al. Monatshefte für Chemie (2005) 136: 367. doi:10.1007/s00706-004-0276-5

Summary.

In collaboration with Jasco Corporation we have recently developed an FDCD (fluorescence detected circular dichroic) instrument J-465, which eliminates the photoselection artifacts and efficiently collects the emitted light from the sample solution based on the ideal ellipsoidal mirror principle. Using the J-465 we have investigated a variety of fluorophores with/without polarization for exciton-coupled FDCD stereochemical analysis. The following three cases extend the FDCD methodology to new challenging areas beyond the limits of conventional CD: (1) substrates containing C=C double bonds, (2) molecules with sterically hindered hydroxyls, and (3) substrates bearing remote stereogenic centers. The pico- to nano-level FDCD analysis described in this paper leads to promising opportunities for the stereochemical analysis of a wide range of natural products with minuscule amounts of sample available.

Keywords. Fluorescence detected exciton coupled CD; Photoselection; Sensitivity enhancement; Cross metathesis; Styrenoid.

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • Katsunori Tanaka
    • 1
  • Gennaro Pescitelli
    • 2
  • Koji Nakanishi
    • 1
  • Nina Berova
    • 1
  1. 1.Department of ChemistryColumbia UniversityNew YorkUSA
  2. 2.Dipartimento di Chimica e Chimica IndustrialeUniversità degli Studi di PisaPisaItaly