Archives of Virology

, Volume 146, Issue 2, pp 293–302

Immune responses and protection induced by mucosal and systemic immunisation with recombinant measles nucleoprotein in a mouse model of measles virus-induced encephalitis

  • W. Olszewska
  • J. Erume
  • J. Ripley
  • M. W. Steward
  • C. D. Partidos

DOI: 10.1007/s007050170176

Cite this article as:
Olszewska, W., Erume, J., Ripley, J. et al. Arch. Virol. (2001) 146: 293. doi:10.1007/s007050170176

Summary.

 In this study the immunogenicity of recombinant nucleoprotein (Np) administered intranasally or intraperitoneally, and its ability to support a systemic protective anti-virus antibody response was examined, in a mouse model of measles virus (MV)-induced encephalitis. Although both intranasal and intraperitoneal routes of immunisation resulted in priming Np-and MV-specific T-cell responses, the intraperitoneal route was shown to prime for a predominantly IgG2a serum anti-MV antibody response of high avidity, which confered complete protection following intracranial challenge with a neuroadapted strain of MV. On the other hand, intranasal priming resulted in a mixed IgG1, IgG2a serum anti-MV antibody response of low avidity, and only 43% of immunised mice survived following intracranial challenge with the neuroadapted strain of MV.

These findings suggest that the route of immunisation in combination with an appropriate adjuvant could influence the induction of a quality antibody response with protective capacity.

Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • W. Olszewska
    • 1
  • J. Erume
    • 2
  • J. Ripley
    • 1
  • M. W. Steward
    • 1
  • C. D. Partidos
    • 2
  1. 1. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, U.K.GB
  2. 2. Department of Pathology and Infectious Diseases, The Royal Veterinary College, London, U.K.GB