Archives of Virology

, Volume 146, Issue 9, pp 1753–1763

Study of the polymerization step of the rolling circle replication of peach latent mosaic viroid

  • M. Pelchat
  • F. Coté
  • J.-P. Perreault
Brief Report

DOI: 10.1007/s007050170061

Cite this article as:
Pelchat, M., Coté, F. & Perreault, JP. Arch. Virol. (2001) 146: 1753. doi:10.1007/s007050170061

Summary.

 We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to the 5′-ends of two small (280 nt) PLMVd-related RNAs found in infected peach leaves. Using a series of truncated PLMVd-derived transcripts, we have demonstrated that the viroid domain composed solely of the self-complementary hammerhead sequences is sufficient to trigger polymerase-driven replication in vitro. These data suggest that the bacterial-like RNA polymerase from peach chloroplasts catalyzes PLMVd replication.

Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • M. Pelchat
    • 1
  • F. Coté
    • 1
  • J.-P. Perreault
    • 1
  1. 1.Département de Biochimie, Faculté de Médecine, Université de Sherbooke, Sherbrooke, CanadaCA