Brief Report

Archives of Virology

, Volume 146, Issue 11, pp 2239-2247

First online:

Cultures of equine respiratory epithelial cells and organ explants as tools for the study of equine influenza virus infection

  • C. LinAffiliated withThe Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, U.S.A.
  • , R. E. Holland JrAffiliated withThe Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, U.S.A.
  • , N. M. WilliamsAffiliated withThe Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, U.S.A.
  • , T. M. ChambersAffiliated withThe Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, U.S.A.

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Summary.

 Equine nasal turbinate epithelial cells and tracheal rafts were maintained with sustained viability in culture. Both types of culture supported productive replication of equine influenza virus (equine-2, subtype H3N8) and cell death occurred through apoptosis following viral infection. Thus, primary respiratory epithelial cell and organ cultures of equine origin may be valuable as alternatives to the intact animal for studying the virus-host interaction of equine respiratory viruses including influenza.