Archives of Virology

, Volume 146, Issue 12, pp 2469–2479

Identification of epitopes on the envelope (E) protein of dengue 2 and dengue 3 viruses using monoclonal antibodies

  • I. L. Serafin
  • J. G. Aaskov
Brief Report

DOI: 10.1007/s007050170017

Cite this article as:
Serafin, I. & Aaskov, J. Arch. Virol. (2001) 146: 2469. doi:10.1007/s007050170017

Summary.

 Of a panel of forty-six anti-dengue 3 monoclonal antibodies (MAbs) only three neutralised infection of BHK cells by dengue 3 virus. Attempts to select neutralisation escape mutants (n.e.m.) with two of these antibodies failed. The n.e.m. population selected in the presence of the third neutralising antibody, 1H9, had a nucleotide change at position 1157 of the E protein gene resulting in a non-conservative amino acid change at E386 for a Lys to an Asn. A dengue 2 n.e.m. was selected with the flavivirus crossreactive IgG monoclonal antibody 4G2, had deduced amino acid changes at E169 (Ser to Pro) and E275 (Gly to Arg). This dengue 2 n.e.m. population produced smaller plaques in BHK cells than the parental virus, decreased fusion activity (FFWI) and had lost the ability to agglutinate gander erythrocyes at pH 6.0 to 6.7.

Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • I. L. Serafin
    • 1
  • J. G. Aaskov
    • 1
  1. 1.Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Queensland, AustraliaAU