Sequence and initial characterization of the UL10 (glycoprotein M) and UL11 homologous genes of serotype 1 Marek’s Disease Virus
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- Osterrieder, N. Arch. Virol. (1999) 144: 1853. doi:10.1007/s007050050710
The nucleotide sequence of the UL10 (glycoprotein M) and the UL11 homologs of Marek’s Disease Virus 1 strain GA was determined. The UL10 open reading frame encodes a type III membrane protein of 424 amino acids that contains eight hydrophobic domains and two consensus N-linked glycosylation sites. The UL11 homologous gene encodes an 84 amino acid polypeptide, and contains a highly conserved myristylation site at its aminoterminus. By analysis of infected-cell RNA with strand-specific RNA probes, transcription of both UL10 and UL11 in infected cells was demonstrated. Coupled in vitro transcription-translation confirmed that the UL10 product is a 47 kD N-glycosylated viral protein that aggregated upon boiling, whereas the UL11 protein exhibited a size of 12 kD after in vitro translation.