Archives of Virology

, Volume 142, Issue 5, pp 939–952

Involvement of dsRNA virus in the protein compositionand growth kinetics of host Trichomonas vaginalis

  • D. Provenzano
  • A. Khoshnan
  • J. F. Alderete
Article

DOI: 10.1007/s007050050130

Cite this article as:
Provenzano, D., Khoshnan, A. & Alderete, J. Arch. Virol. (1997) 142: 939. doi:10.1007/s007050050130

Summary

Trichomonas vaginalis harbors a double-stranded (ds)-RNA virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (Khoshnan A, Alderete JF (1994) J Virol 68: 4 035–4 038). To further test the influence of virus on T. vaginalis, virus-infected (V+) isolates were compared to virus-free (V-), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. Comparative high resolution two dimensional (2D)-SDS-PAGE was performed of trichomonads grown in a chemostat under identical conditions. At least 47 proteins were identified as specifically expressed by representative V+ isolate 347, and ∼41 spots were specific to the corresponding V- progeny, showing an association between virus and the presence and absence of parasite proteins. Qualitatively and quantitatively dissimilar cysteine proteinase patterns were detected from numerous V+ isolates and the V- progeny. A 2D analysis for isolate 347 showed the appearance of unique proteinase activities for parental parasites and presence of at least one proteinase in the V- progeny. Finally, the V+T. vaginalis isolate 347, but not the V- isolate 347 progeny nor other V+ isolates, underwent fluctuations in density during chemostat growth allowing for purification of virus particles from the V+ isolate 347 supernatants during decreased parasite density.

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • D. Provenzano
    • 1
  • A. Khoshnan
    • 2
  • J. F. Alderete
    • 1
  1. 1.Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, U.S.AUS
  2. 2.Department of Microbiology, University ofSouthern California, Los Angeles, California, U.S.A.US