Archives of Virology

, Volume 142, Issue 4, pp 853–860

Nucleotide sequencing of a part of the 5′-noncoding region of echovirus type 9 and rapid virus detection during the acute phase of aseptic meningitis

  • G. R. Kim
  • J. S. Lee
  • Y. T. Jung
  • Y. J. Chung
  • M. G. Rhyu
Brief Report

DOI: 10.1007/s007050050124

Cite this article as:
Kim, G.R., Lee, J.S., Jung, Y.T. et al. Arch. Virol. (1997) 142: 853. doi:10.1007/s007050050124

Summary

A part of the 5′-noncoding region of echovirus type 9 isolates was sequenced, and an attempt was made for rapid virus detection in clinical samples obtained from 22 subjects hospitalized with aseptic meningitis. The sequence identity of 440-bp products amplified from the region by RT-PCR was 87.7% between the standard echovirus type 9(Hill strain) and the isolates. Specific IgM antibodies to Hill strain were positive in 45.5% by immunofluorescent antibody staining of virus-infected cells. A high detection rate of PCR products was observed in cerebrospinal fluids (CSFs; 54.5%) at admission, and in peripheral mononuclear cells (PMCs; 72.7%) at the end of hospitalization. Viral genomes were detectable for 2 days in serum samples, and for 6 days in PMC samples after onset of disease. When specific IgM antibody titers were less than 1:40, the amplification rate of viral genome from serum samples was 50.0%. These results indicate that the combination of specific IgM determination and viral genome amplification from CSFs will be a rapid and reliable method for early diagnosis.

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • G. R. Kim
    • 1
  • J. S. Lee
    • 2
  • Y. T. Jung
    • 1
  • Y. J. Chung
    • 1
  • M. G. Rhyu
    • 1
  1. 1.Department of Microbiology, Catholic University, Medical College, Seoul, KoreaKorea
  2. 2.Department of Pediatrics, Catholic University, Medical College, Seoul, KoreaKorea

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