Archives of Virology

, Volume 145, Issue 3, pp 553–565

Grapevine fleck virus-like viruses in Vitis

Authors

  • S. Sabanadzovic
    •  Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy
  • N. Abou-Ghanem
    •  Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy
  • M. A. Castellano
    •  Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy
  • M. Digiaro
    •  Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy
  • G. P. Martelli
    •  Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy

DOI: 10.1007/s007050050046

Cite this article as:
Sabanadzovic, S., Abou-Ghanem, N., Castellano, M. et al. Arch. Virol. (2000) 145: 553. doi:10.1007/s007050050046

Summary.

 Two sets of degenerate primers for the specific amplification of 572–575 nt and 386 nt segments of the methyltransferase and RNA-dependent RNA polymerase cistrons of members of the genera Tymovirus and Marafivirus and of the unassigned virus Grapevine fleck virus (GFkV) were designed on the basis of available sequences. These primers were used for amplifying and subsequent cloning and sequencing part of the open reading frame 1 of the genome of GFkV, Grapevine asteroid mosaic-associated virus (GAMaV) and of another previously unreported virus, for which the name Grapevine red globe virus (GRGV) is proposed. Computer-assisted analysis of the amplified genome portions showed that the three grapevine viruses are phylogenetically related with one another and with sequenced tymoviruses and marafiviruses. The relationships with tymoviruses was confirmed by the type of ultrastructural modifications induced in the host cells. RdRp-specific degenerate primers were successfully used for the aspecific detection of the three viruses in crude grapevine sap extracts. Specific virus identification was obtained with RT-PCR using antisense virus-specific primers.

Copyright information

© 2000 Springer-Verlag/ Wien