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First description of hemagglutination by a virus belonging to the family Dicistroviridae

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Abstract

Triatoma virus is the only virus whose genome has been sequenced and studied in triatomines. It belongs to the family Dicistroviridae. In order to detect whether TrV has the ability to agglutinate erythrocytes of domestic and laboratory animals, we performed a hemagglutination assay. Positive hemagglutination was found for red blood cells of guinea pigs. The HA assay could be used as a titration method, at least for purified viral particles obtained from triatomine stool. This is the first record of hemagglutinating properties for Dicistroviridae.

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References

  1. Agirre J, Aloria K, Arizmendi J, Iloro I, Elortza F, Sánchez-Eugenia R, Marti G, Neumann E, Rey F, Guerin D (2011) Capsid protein identification and analysis of mature Triatoma virus (TrV) virions and naturally occurring empty particles. Virology 409:91–101

    Article  CAS  PubMed  Google Scholar 

  2. Berquist KR, Fritts SP (1968) Hemagglutinating activity of enteroviruses recovered in two primary cell lines and a stable line. Appl Microbiol 16:703–707

    CAS  PubMed Central  PubMed  Google Scholar 

  3. Bonning BC, Miller AW (2010) Dicistroviruses. Annu Rev Entomol 55:129–150

    Article  CAS  PubMed  Google Scholar 

  4. Czibener C, La Torre JL, Muscio OA, Ugalde RA, Scodeller EA (2000) Nucleotide sequence analysis of Triatoma virus shows that it is a member of a novel group of insect RNA viruses. J Gen Virol 81:149–1154

    Google Scholar 

  5. Erlandson M (2008) Insect pest control by viruses. Encyclopedia of virology, 3rd edn. Elsevier Ltd, Amsterdam

    Google Scholar 

  6. Gorman WL, Pridgen C, Portner A (1991) Glycosylation of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 1 affects its functional but not its antigenic properties. Virology 183:83–90

    Article  CAS  PubMed  Google Scholar 

  7. International Committee of Taxonomy of Viruses, Index (2012)

  8. Killian ML (2008) Hemagglutination assay for the avian influenza virus. Methods Mol Biol 436:47–52

    CAS  PubMed  Google Scholar 

  9. Marti G, Gonzalez T, García J, Viguera A, Guérin D, Echeverria M (2008) AC-ELISA and RT-PCR assays for diagnosis of triatoma virus (TrV) in triatomines (Hemiptera: Reduviidae) species. Arch Virol 153:1427–1432

    Article  CAS  PubMed  Google Scholar 

  10. Masoumi A, Hanzlik TN, Christian PD (2003) Functionality of the 5′—and intergenic IRES elements of cricket paralysis virus in a range of insect cell lines, and its relationship with viral activities. Virus Res 94:113–120

    Article  CAS  PubMed  Google Scholar 

  11. Moore N, King L, Pullin J (1987) Insect picornaviruses. In: The Molecular biology of the positive strand RNA viruses. Academic Press, London, pp 17–43

  12. Moore N, Reavey B, King L (1985) General characteristics, gene organization and expression of small RNA viruses of insects. J Gen Virol 66:647–659

    Article  CAS  Google Scholar 

  13. Muscio OA, La Torre JL, Scodeller EA (1988) Characterization of Triatoma virus, a picorna-like virus isolated from the triatomine bug Triatoma infestans. J Gen Virol 69:2929–2934

    Article  CAS  PubMed  Google Scholar 

  14. Seago J, Jackson T, Doel C, Fry E, Stuart D, Harmsen MM, Charlestonand B, Juleff N (2012) Characterization of epitope-tagged foot-and-mouth disease virus. J Gen Virol 93:2371–2381

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Shimabukuro K, Ujike M, Ito T, Tsunemitsu H, Oshitani H, Taguchi F (2013) Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus. Arch Virol 158:1561–1566

    Article  CAS  PubMed  Google Scholar 

  16. Smith TJ, Brandt WE, Swanson JL, McCown JM, Buescher EL (1970) Physical and biological properties of dengue-2 virus and associated antigens. J Virol 5:524–532

    CAS  PubMed Central  PubMed  Google Scholar 

  17. World Health Organization (2010) Working to overcome the global impact of neglected tropical diseases. First WHO report on neglected tropical diseases

Download references

Acknowledgments

This study was partially funded by the following grants: PIP 0007 (CONICET) and PICT 2011-1081 (ANPCyT), Argentina. The authors thank the Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina and CEPAVE (Centro de Estudios Parasitológicos y de Vectores), where this work was carried out. Thanks are also extended to Cátedra de Producción, Facultad de Agronomía, UNLP; Cátedra de Zootecnia II; Zootecnia I; Hospital de Clínicas; Cátedra de Animales de Laboratorio, Facultad de Ciencias Veterinarias, UNLP, Zoológico de La Plata, and Drs. Alejandro Valera and Marcelo Pecoraro.

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Authors and Affiliations

  1. Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT-La Plata-CONICET-UNLP), 2 #584, 1900, La Plata, Argentina

    María Laura Susevich & Gerardo Aníbal Marti

  2. CONICET (CCT-La Plata), La Plata, Argentina

    María Laura Susevich, Gerardo Aníbal Marti & María Gabriela Echeverría

  3. Virology Department, Faculty of Veterinary Sciences (UNLP), 60 y 118, 1900, La Plata, Argentina

    María Gabriela Echeverría

Authors
  1. María Laura Susevich
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  2. Gerardo Aníbal Marti
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  3. María Gabriela Echeverría
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Correspondence to María Gabriela Echeverría.

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Susevich, M.L., Marti, G.A. & Echeverría, M.G. First description of hemagglutination by a virus belonging to the family Dicistroviridae . Arch Virol 159, 581–584 (2014). https://doi.org/10.1007/s00705-013-1865-6

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  • DOI: https://doi.org/10.1007/s00705-013-1865-6

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