Archives of Virology

, Volume 159, Issue 3, pp 509–518

Investigating a crow die-off in January–February 2011 during the introduction of a new clade of highly pathogenic avian influenza virus H5N1 into Bangladesh

  • Salah Uddin Khan
  • LaShondra Berman
  • Najmul Haider
  • Nancy Gerloff
  • Md Z. Rahman
  • Bo Shu
  • Mustafizur Rahman
  • Tapan Kumar Dey
  • Todd C. Davis
  • Bidhan Chandra Das
  • Amanda Balish
  • Ausraful Islam
  • Jens P. Teifke
  • Nord Zeidner
  • Steven Lindstrom
  • Alexander Klimov
  • Ruben O. Donis
  • Stephen P. Luby
  • H. L. Shivaprasad
  • Andrea B. Mikolon
Original Article

DOI: 10.1007/s00705-013-1842-0

Cite this article as:
Khan, S.U., Berman, L., Haider, N. et al. Arch Virol (2014) 159: 509. doi:10.1007/s00705-013-1842-0

Abstract

We investigated unusual crow mortality in Bangladesh during January-February 2011 at two sites. Crows of two species, Corvus splendens and C. macrorhynchos, were found sick and dead during the outbreaks. In selected crow roosts, morbidity was ~1 % and mortality was ~4 % during the investigation. Highly pathogenic avian influenza virus H5N1 clade 2.3.2.1 was isolated from dead crows. All isolates were closely related to A/duck/India/02CA10/2011 (H5N1) with 99.8 % and A/crow/Bangladesh/11rs1984-15/2011 (H5N1) virus with 99 % nucleotide sequence identity in their HA genes. The phylogenetic cluster of Bangladesh viruses suggested a common ancestor with viruses found in poultry from India, Myanmar and Nepal. Histopathological changes and immunohistochemistry staining in brain, pancreas, liver, heart, kidney, bursa of Fabricius, rectum, and cloaca were consistent with influenza virus infection. Through our limited investigation in domesticated birds near the crow roosts, we did not identify any samples that tested positive for influenza virus A/H5N1. However, environmental samples collected from live-bird markets near an outbreak site during the month of the outbreaks tested very weakly positive for influenza virus A/H5N1 in clade 2.3.2.1-specific rRT-PCR. Continuation of surveillance in wild and domestic birds may identify evolution of new avian influenza virus and associated public-health risks.

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Salah Uddin Khan
    • 1
    • 2
  • LaShondra Berman
    • 3
  • Najmul Haider
    • 1
  • Nancy Gerloff
    • 3
  • Md Z. Rahman
    • 1
  • Bo Shu
    • 3
  • Mustafizur Rahman
    • 1
  • Tapan Kumar Dey
    • 4
  • Todd C. Davis
    • 3
  • Bidhan Chandra Das
    • 5
  • Amanda Balish
    • 3
  • Ausraful Islam
    • 1
  • Jens P. Teifke
    • 6
  • Nord Zeidner
    • 1
    • 3
  • Steven Lindstrom
    • 3
  • Alexander Klimov
    • 3
  • Ruben O. Donis
    • 3
  • Stephen P. Luby
    • 1
    • 3
  • H. L. Shivaprasad
    • 7
  • Andrea B. Mikolon
    • 1
    • 3
  1. 1.CCD, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B)DhakaBangladesh
  2. 2.Department of Environmental and Global Health, College of Public Health and Health ProfessionsUniversity of FloridaGainesvilleUSA
  3. 3.Centers for Disease Control and Prevention, (CDC)AtlantaUSA
  4. 4.Forest Department, Ministry of Environment and ForestDhakaBangladesh
  5. 5.Department of Livestock ServicesMinistry of Fisheries and LivestockDhakaBangladesh
  6. 6.Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal HealthGreifswald-Insel RiemsGermany
  7. 7.California Animal Health and Food Safety Laboratory System, Tulare branchUniversity of California, DavisTulareUSA