Archives of Virology

, Volume 159, Issue 2, pp 371–373

Existence of feline morbillivirus infection in Japanese cat populations

  • Tetsuya Furuya
  • Yukiko Sassa
  • Tsutomu Omatsu
  • Makoto Nagai
  • Ryuji Fukushima
  • Makoto Shibutani
  • Tomohiro Yamaguchi
  • Yosuke Uematsu
  • Kinji Shirota
  • Tetsuya Mizutani
Rapid Communication

DOI: 10.1007/s00705-013-1813-5

Cite this article as:
Furuya, T., Sassa, Y., Omatsu, T. et al. Arch Virol (2014) 159: 371. doi:10.1007/s00705-013-1813-5

Abstract

Feline morbillivirus (FmoPV) is a member of a new virus species that has only been found in the Hong Kong cat population. For the first time, however, we have now detected nucleotide sequences similar to FmoPV in samples from Japanese cat populations. The positive rates for urine and blood samples from Japanese cats were 6.1 % (5/82) and 10 % (1/10), respectively. These sequences are similar to the previously reported FmoPV, with 92-94 % identity, and substantially different from all other morbilliviruses. Phylogenetic analysis of the identified Japanese FmoPVs and other morbilliviruses demonstrated a pattern similar to those previously published for the FmoPV viruses isolated in Hong Kong. FmoPV RNA was also detected from formalin-fixed paraffin-embedded (FFPE) kidney tissues of cats with nephritis, with a positive rate of 40 % (4/10). By using nested-set primers based on the FmoPV sequence and RNA from FFPE tissues, we demonstrated the existence of FmoPV infection in Japanese cats and established the method for detection of the FmoPV RNA from kidney tissues prepared for pathology examinations, which is useful for studies on the pathogenicity of the virus.

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Tetsuya Furuya
    • 1
    • 2
  • Yukiko Sassa
    • 1
    • 3
  • Tsutomu Omatsu
    • 1
  • Makoto Nagai
    • 1
    • 3
  • Ryuji Fukushima
    • 4
  • Makoto Shibutani
    • 5
  • Tomohiro Yamaguchi
    • 6
  • Yosuke Uematsu
    • 6
  • Kinji Shirota
    • 7
  • Tetsuya Mizutani
    • 1
  1. 1.Research and Education center for Prevention of Global Infectious Diseases of AnimalsTokyo University of Agriculture and TechnologyTokyoJapan
  2. 2.Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of AgricultureTokyo University of Agriculture and TechnologyTokyoJapan
  3. 3.Laboratory of Epizootiology, Department of Veterinary Medicine, Faculty of AgricultureTokyo University of Agriculture and TechnologyTokyoJapan
  4. 4.Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of AgricultureTokyo University of Agriculture and TechnologyTokyoJapan
  5. 5.Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of AgricultureTokyo University of Agriculture and TechnologyTokyoJapan
  6. 6.Canine-Lab. Inc.TokyoJapan
  7. 7.Laboratory of Veterinary Pathology, School of Veterinary MedicineAzabu UniversityKanagawaJapan