Archives of Virology

, Volume 158, Issue 3, pp 717–721

Analysis of molecular variation of porcine reproductive and respiratory syndrome virus in Central China from 2006 to 2012

  • Yuejun Shi
  • Zhibin Hu
  • Zhenghui Xiong
  • Yanjun Zhou
  • Xin Jin
  • Changqin Gu
  • Xueying Hu
  • Guofu Cheng
  • Nianhua Song
  • Wanpo Zhang
Annotated Sequence Record

DOI: 10.1007/s00705-012-1542-1

Cite this article as:
Shi, Y., Hu, Z., Xiong, Z. et al. Arch Virol (2013) 158: 717. doi:10.1007/s00705-012-1542-1
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Abstract

To analyze the epidemiology of PRRSV in Hubei Province of China, 668 serum samples collected from 14 pig-breeding farms were tested. We found that the PRRSV-positive rate was 5.24 % and that HP-PRRSV had become the dominant strain. To further investigate the genetic variation of PRRSV strains in this region, the complete gene sequences of nsp2, orf5, and orf7 from nine PRRSV strains collected during 2011-2012 were determined and compared with 33 known sequences. The results revealed that diverse HP-PRRSV strains are present in this region. An analysis of orf5 gene sequences showed that the strains collected during 2009-2010 formed a tightly clustered branch. When compared with the JXA1 strain, they had one mutation (V29 → A29) in a decoy epitope. Furthermore, we found that the number of potential N-glycosylation sites had apparently increased since 2006. These findings increase our knowledge of PRRSV epidemiology in Central China.

Supplementary material

705_2012_1542_MOESM1_ESM.pdf (33 kb)
Geographic origin and amplified gene sequences of the samples in this study (PDF 32 kb)
705_2012_1542_MOESM2_ESM.pdf (80 kb)
Primers used for amplification of the nsp2, orf5 and orf7 genes (PDF 79 kb)
705_2012_1542_MOESM3_ESM.pdf (39 kb)
Origin of PRRSV strains downloaded from GenBank database (PDF 39 kb)
705_2012_1542_MOESM4_ESM.pdf (357 kb)
Alignment based on the deduced amino acid sequences of the nsp2 genes (PDF 356 kb)
705_2012_1542_MOESM5_ESM.pdf (600 kb)
Alignment based on the deduced amino acid sequences of the orf5 genes (PDF 599 kb)
705_2012_1542_MOESM6_ESM.pdf (161 kb)
Alignment based on the deduced amino acid sequences of the orf7 genes (PDF 160 kb)

Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Yuejun Shi
    • 1
  • Zhibin Hu
    • 2
  • Zhenghui Xiong
    • 1
  • Yanjun Zhou
    • 2
  • Xin Jin
    • 2
  • Changqin Gu
    • 1
  • Xueying Hu
    • 1
  • Guofu Cheng
    • 1
  • Nianhua Song
    • 2
  • Wanpo Zhang
    • 1
  1. 1.College of Veterinary MedicineHuazhong Agricultural UniversityWuhanChina
  2. 2.Hubei Center for Animal Disease Control and PreventionWuhanChina