Archives of Virology

, Volume 158, Issue 1, pp 165–172

Hepatitis E virus genotype 4 isolated from a patient with liver failure: full-length sequence analysis showing potential determinants of virus pathogenesis

  • Qiuning Bu
  • Xiaojuan Wang
  • Ling Wang
  • Peng Liu
  • Jiabao Geng
  • Maorong Wang
  • Jian Han
  • Yonghong Zhu
  • Hui Zhuang
Original Article

DOI: 10.1007/s00705-012-1488-3

Cite this article as:
Bu, Q., Wang, X., Wang, L. et al. Arch Virol (2013) 158: 165. doi:10.1007/s00705-012-1488-3

Abstract

The full-length genome sequence of a genotype 4 strain of Hepatitis E virus (HEV) (CHN-NJ-H2011) from a patient (in Nanjing, China) with liver failure has been determined. Phylogenetic analysis showed that CHN-NJ-H2011 belongs to genotype 4, subtype 4h. Comparative sequence analysis carried out on a 301-bp fragment of ORF2 showed that CHN-NJ-H2011 shares high nucleotide sequence identity (94.3–94.7 %) with porcine viruses (ch-shsw1 and Ch-estw2) isolated in the same geographical region, pointing to the strong possibility of zoonotic transmission of HEV genotype 4. A broader comparison with other genotype 4 isolates revealed 12 unique amino acid substitutions in ORF1 and three in ORF2 that might serve as signatures of disease severity for genotype 4 HEV infection.

Copyright information

© Springer-Verlag Wien 2012

Authors and Affiliations

  • Qiuning Bu
    • 1
    • 3
  • Xiaojuan Wang
    • 1
  • Ling Wang
    • 1
  • Peng Liu
    • 1
  • Jiabao Geng
    • 2
  • Maorong Wang
    • 2
  • Jian Han
    • 1
  • Yonghong Zhu
    • 1
  • Hui Zhuang
    • 1
  1. 1.Department of Microbiology, School of Basic Medical Sciences, Peking University Infectious Disease CenterPeking UniversityBeijingChina
  2. 2.Institute of HepatologyThe 81st Hospital of PLANanjingChina
  3. 3.Clinical LaboratoryThe First Hospital of QinhuangdaoQinhuangdaoChina