Archives of Virology

, Volume 158, Issue 1, pp 193–199

Human papillomavirus type 58 L1 virus-like particles purified by two-step chromatography elicit high levels of long-lasting neutralizing antibodies

Authors

  • Xixiu Xie
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Yanchun Liu
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Ting Zhang
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Yanying Xu
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Qifeng Bao
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Xue Chen
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
  • Hongyang Liu
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
    • Department of Biophysics and Structural BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College
Brief Report

DOI: 10.1007/s00705-012-1465-x

Cite this article as:
Xie, X., Liu, Y., Zhang, T. et al. Arch Virol (2013) 158: 193. doi:10.1007/s00705-012-1465-x

Abstract

Human papillomavirus (HPV) type 58 is a high-risk type of HPV frequently detected in cervical cancers, especially in Eastern Asia. There are still no commercially available vaccines against HPV 58 infection. High levels of long-lasting neutralizing antibodies are crucial for long-term protection against HPV infection. Here, we have developed a two-step chromatography strategy and have purified highly pure HPV L1 proteins, which form more homogenous and uniform VLPs than those purified by CsCl ultracentrifugation. Low-dosage immunization with HPV 58 L1 VLPs alone or co-administrated with HPV 16 and HPV 18 L1 VLPs is sufficient to induce high levels of long-lasting neutralizing antibodies in mice. Our results suggest that the highly immunogenic HPV 58 L1 VLPs are a good candidate for use in developing effective vaccines against HPV 58 infection.

Supplementary material

705_2012_1465_MOESM1_ESM.pdf (129 kb)
Supplementary material 1 (PDF 129 kb)

Copyright information

© Springer-Verlag 2012