Archives of Virology

, Volume 157, Issue 12, pp 2431–2435

Bacteriophage vB_EcoM_FV3: a new member of “rV5-like viruses”

  • Lidija Truncaite
  • Eugenijus Šimoliūnas
  • Aurelija Zajančkauskaite
  • Laura Kaliniene
  • Roma Mankevičiūte
  • Juozas Staniulis
  • Vytautas Klausa
  • Rolandas Meškys
Brief Report

DOI: 10.1007/s00705-012-1449-x

Cite this article as:
Truncaite, L., Šimoliūnas, E., Zajančkauskaite, A. et al. Arch Virol (2012) 157: 2431. doi:10.1007/s00705-012-1449-x

Abstract

A proposed new genus of the family Myoviridae, “rV5-like viruses”, includes two lytic bacteriophages: Escherichia coli O157: H7-specific bacteriophage rV5 and Salmonella phage PVP-SE1. Here, we present basic properties and genomic characterization of a novel rV5-like phage, vB_EcoM_FV3, which infects E. coli K-12-derived laboratory strains and replicates at high temperature (up to 47 °C). The 136,947-bp genome of vB_EcoM_FV3 contains 218 open reading frames and encodes 5 tRNAs. The genomic content and organization of vB_EcoM_FV3 is more similar to that of rV5 than to PVP-SE1, but all three phages share similar morphological characteristics and form a homogeneous phage group.

Keywords

Escherichia colirV5-like virusesBacteriophage vB_EcoM_FV3High temperature

Supplementary material

705_2012_1449_MOESM1_ESM.doc (286 kb)
Supplementary material 1 Table 1S. A list of bacteriophage vB_EcoM_FV3 putative genes with predicted functions. (DOC 285 kb)
705_2012_1449_MOESM2_ESM.tif (4.6 mb)
Supplementary material 2 Fig. 1S. Functional genomic map of bacteriophage vB_EcoM_FV3. Putative genes with predicted coding functions are represented as arrows. The colour code for the online version of the article is as follows: yellow—DNA replication, recombination, repair and packaging; brown—transcription, translation, nucleotide metabolism; blue—virion structure; green—chaperones/assembly; purple—lysis, host or phage interactions; grey—ORFs of unknown function; red—unique FV3 ORFs of unknown function; black—tRNA. (TIFF 4748 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Lidija Truncaite
    • 1
  • Eugenijus Šimoliūnas
    • 1
  • Aurelija Zajančkauskaite
    • 1
  • Laura Kaliniene
    • 1
  • Roma Mankevičiūte
    • 1
  • Juozas Staniulis
    • 2
  • Vytautas Klausa
    • 1
  • Rolandas Meškys
    • 1
  1. 1.Department of Molecular Microbiology and BiotechnologyInstitute of Biochemistry of Vilnius UniversityVilniusLithuania
  2. 2.Laboratory of Plant VirusesInstitute of Botany of the Nature Research CentreVilniusLithuania