Archives of Virology

, Volume 157, Issue 7, pp 1339–1344

Identification of three new type-specific antigen epitopes in the capsid protein of porcine circovirus type 1

  • Liping Huang
  • Yuehua Lu
  • Yanwu Wei
  • Longjun Guo
  • Changming Liu
Brief Report

DOI: 10.1007/s00705-012-1268-0

Cite this article as:
Huang, L., Lu, Y., Wei, Y. et al. Arch Virol (2012) 157: 1339. doi:10.1007/s00705-012-1268-0

Abstract

Porcine circovirus type 1 (PCV1) has been identified as a contaminant of porcine kidney cell line (PK-15). Serological evidence and genetic studies have suggested that PCV1 is widespread in domestic pigs. In this study, monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) were generated against a recombinant PCV1 Cap protein (PCV1-Cap), which was expressed using the baculovirus system. PEPSCAN analysis was used to identify epitopes on the PCV1-Cap with mAbs and pAbs. Three linear B-cell epitopes, including residues 85GGTNPLP91, 162FTPKPELDKTIDWFHPNNK180 and 219YVQFREFILKDPLNK233, specific for PCV1-Cap, were finely defined. These results will facilitate future investigations into antigenic differences and differential diagnosis between PCV1 and PCV2.

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Liping Huang
    • 1
  • Yuehua Lu
    • 1
  • Yanwu Wei
    • 1
  • Longjun Guo
    • 1
  • Changming Liu
    • 1
  1. 1.Division of Swine Infectious Diseases, State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research Institute, The Chinese Academy of Agricultural SciencesHarbinChina