Annotated Sequence Record

Archives of Virology

, Volume 157, Issue 2, pp 383-385

First online:

Complete sequence of a cryptic virus from hemp (Cannabis sativa)

  • Angelika ZieglerAffiliated withInstitute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute Email author 
  • , Jaroslav MatoušekAffiliated withBiol. Centre AS CR v.v.i., Institute of Plant Molecular Biology
  • , Gerhard StegerAffiliated withDepartment of Biophysics, Heinrich-Heine-University
  • , Jörg SchubertAffiliated withInstitute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute

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Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus. The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained.