Archives of Virology

, Volume 156, Issue 12, pp 2215–2220

Complete genome sequences and phylogenetic analysis of hepatitis delta viruses isolated from nine Turkish patients

Authors

  • İnci Çelik
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
  • Ersin Karataylı
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
  • Emrah Çevik
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
  • S. Gökçe Kabakçı
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
  • Senem Ceren Karataylı
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
  • Bedia Dinç
    • Microbiology DepartmentYüksek İhtisas Hospital
  • Kubilay Çınar
    • Gastroenterology Department, School of MedicineAnkara University
  • Kendal Yalçın
    • Gastroenterology Department, School of MedicineDicle University
  • Ramazan İdilman
    • Gastroenterology Department, School of MedicineAnkara University
  • Cihan Yurdaydın
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
    • Gastroenterology Department, School of MedicineAnkara University
    • Department of Gastroenterology, Institute of Hepatology, School of MedicineAnkara University
    • Gastroenterology Department, School of MedicineAnkara University
Original Article

DOI: 10.1007/s00705-011-1120-y

Cite this article as:
Çelik, İ., Karataylı, E., Çevik, E. et al. Arch Virol (2011) 156: 2215. doi:10.1007/s00705-011-1120-y

Abstract

Hepatitis delta virus (HDV) is a subviral agent of hepatitis B virus (HBV), and its life cycle is dependent on HBV. It is commonly accepted that HDV has eight distinct genotypes. In this study, the complete nucleotide sequences of HDV genomes isolated from nine Turkish patients were obtained by RT-PCR using two pairs of primers that cover the entire HDV genome. PCR products were sequenced directly. The results showed that these 9 isolates were approximately 1680 base pairs in length and clustered in the genotype HDV-1 branch when phylogenetic analysis was done with the sequences together with the complete sequences of HDV genomes representing each genotype retrieved from GenBank. Analysis of a portion of the large hepatitis D antigen (L-HDAg) gene showed that sequence similarity among these Turkish isolates is between 87.4 and 97.1%, and the Turkish isolates have the most sequence similarity to HDV-1 (90.5%), while they have the least sequence similarity to HDV-3 (64.1%). Full-genome analysis indicates that the sequence similarity is between 80.7 and 95.4%, and the highest sequence similarity is 84.8% (between the Turkish isolates and HDV-1). The lowest sequence similarity is 56.4% (between the Turkish isolates and HDV-3). In conclusion, phylogenetic analysis shows that the Turkish HDV isolates belong to HDV-1.

Copyright information

© Springer-Verlag 2011