Archives of Virology

, 156:1787

Relative reactivity of HIV-1 polyclonal plasma antibodies directed to V3 and MPER regions suggests immunodominance of V3 over MPER and dependence of high anti-V3 antibody titers on virus persistence

  • Raiees Andrabi
  • Alok K. Choudhary
  • Manju Bala
  • Rajesh Kalra
  • S. S. Prakash
  • R. M. Pandey
  • Kalpana Luthra
Original Article

DOI: 10.1007/s00705-011-1053-5

Cite this article as:
Andrabi, R., Choudhary, A.K., Bala, M. et al. Arch Virol (2011) 156: 1787. doi:10.1007/s00705-011-1053-5

Abstract

Antibodies to two crucial regions, the third variable loop (V3) of gp120 and the membrane-proximal external region (MPER) of gp41 are important for HIV-1 neutralization. We here evaluated the relative binding of polyclonal plasma antibodies from 99 HIV-1-infected individuals from India to the consensus–C V3 and MPER peptides and observed immunodominance of V3 over MPER (p < 0.0001). We further examined the V3- and MPER-specific antibody correlates with clinical parameters. Our results revealed that anti-V3 antibody titers are significantly lower in patients on ART compared to drug-naive individuals (p < 0.0001), most likely due to a decrease in plasma viral load, irrespective of their CD4 counts and total IgG. No such association was observed for MPER, with a similar trend in four follow-up patients. These findings strongly suggest that high titers of V3-specific antibodies are dependent on persistence of virus in circulation, while antibodies to MPER are probably not.

Supplementary material

705_2011_1053_MOESM1_ESM.doc (124 kb)
Supplementary material 1 (DOC 124 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Raiees Andrabi
    • 1
  • Alok K. Choudhary
    • 1
  • Manju Bala
    • 2
  • Rajesh Kalra
    • 1
  • S. S. Prakash
    • 1
  • R. M. Pandey
    • 3
  • Kalpana Luthra
    • 1
  1. 1.Department of BiochemistryAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Regional STD Teaching Training and Research CentreSafdarjang HospitalNew DelhiIndia
  3. 3.Department of BiostatisticsAll India Institute of Medical SciencesNew DelhiIndia