Archives of Virology

, Volume 156, Issue 8, pp 1379–1385

Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010

Authors

  • Masayuki Motoshima
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
  • Masatoshi Okamatsu
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
  • Shingo Asakura
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
  • Saya Kuribayashi
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
  • Sugar Sengee
    • State Central Veterinary Laboratory
  • Damdinjav Batchuluun
    • State Central Veterinary Laboratory
  • Mika Ito
    • Ishikawa Nanbu Livestock Hygiene Service Center
  • Yukiko Maeda
    • Ishikari Livestock Hygiene Service Center
  • Mariko Eto
    • Animal Quarantine Service, Ministry of AgricultureForestry and Fisheries
  • Yoshihiro Sakoda
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
  • Ruuragchaa Sodnomdarjaa
    • State Central Veterinary Laboratory
    • Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary MedicineHokkaido University
    • Research Center for Zoonosis ControlHokkaido University
Original Article

DOI: 10.1007/s00705-011-1000-5

Cite this article as:
Motoshima, M., Okamatsu, M., Asakura, S. et al. Arch Virol (2011) 156: 1379. doi:10.1007/s00705-011-1000-5

Abstract

A/equine/Kanazawa/1/2007 (H3N8), A/equine/Hokkaido/I828/2008 (H3N8) and A/equine/Mongolia/1/2008 (H3N8) were isolated from infected horses. A/equine/Yokohama/aq19/2009 (H3N8) and A/equine/Yokohama/aq13/2010 (H3N8) were isolated from horses imported from Canada and Belgium examined at the Animal Quarantine Service in Yokohama, Japan. In the present study, these five isolates were genetically and antigenically analyzed. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed that three isolates from horses in Japan and imported from Canada belonged to the same branch, clade 1 of the Florida sublineage, while the isolates from horses in Mongolia and imported from Belgium belonged to another branch, clade 2 of the Florida sublineage. Reactivity patterns of a panel of monoclonal antibodies to the HA of A/equine/Kanazawa/1/2007 (H3N8) with the five isolates indicate that the HAs of these viruses were antigenically similar to each other and to the reference strains A/equine/La Plata/1/1993 (H3N8) and A/equine/Avesta/1/1993 (H3N8). The present findings indicate that extensive antigenic variation has not accumulated among H3N8 influenza viruses in horses.

Copyright information

© Springer-Verlag 2011