Archives of Virology

, Volume 156, Issue 7, pp 1143–1150

Genetic characterization of a novel calicivirus from a chicken

Original Article

DOI: 10.1007/s00705-011-0964-5

Cite this article as:
Wolf, S., Reetz, J. & Otto, P. Arch Virol (2011) 156: 1143. doi:10.1007/s00705-011-0964-5

Abstract

We describe the identification and genetic characterization of a novel enteric calicivirus, detected by transmission electron microscopy and RT-PCR in two clinically normal chickens and in a chicken with runting and stunting syndrome from different flocks in southern Germany. Positive findings were confirmed by sequencing. The complete nucleotide sequence and genome organization of one strain (Bavaria/04V0021) was determined. The genome of the Bavaria virus is 7,908 nt long and contains two coding open reading frames. Phylogenetic analysis of the deduced partial 2C helicase/NTPase, 3C cysteine protease, RNA-dependent RNA polymerase and complete VP1 capsid protein amino acid sequences showed that the virus is genetically related to but distinct from sapoviruses and lagoviruses. Morphologically, the Bavaria virus particles are 37-42 nm in diameter and exhibit characteristic cup-shaped surface depressions.

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Biology, Institute for MicrobiologyDresden University of TechnologyDresdenGermany
  2. 2.Federal Institute of Risk AssessmentBerlinGermany
  3. 3.Institute for Bacterial Infections and Zoonoses, Friedrich-Loeffler-InstitutFederal Research Institute for Animal HealthJenaGermany