Archives of Virology

, Volume 156, Issue 1, pp 37–51

Pathogenicity of two Egyptian H5N1 highly pathogenic avian influenza viruses in domestic ducks

Authors

  • J. L. Wasilenko
    • Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research LaboratoryUnited States Department of Agriculture, Agricultural Research Service
  • A. M. Arafa
    • National Laboratory for Veterinary Quality Control on Poultry Production
  • A. A. Selim
    • National Laboratory for Veterinary Quality Control on Poultry Production
  • M. K. Hassan
    • National Laboratory for Veterinary Quality Control on Poultry Production
  • M. M. Aly
    • National Laboratory for Veterinary Quality Control on Poultry Production
  • A. Ali
    • Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)
  • S. Nassif
    • Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)
  • E. Elebiary
    • Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)
  • A. Balish
    • Influenza DivisionNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention
  • A. Klimov
    • Influenza DivisionNational Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention
  • D. L. Suarez
    • Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research LaboratoryUnited States Department of Agriculture, Agricultural Research Service
  • D. E. Swayne
    • Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research LaboratoryUnited States Department of Agriculture, Agricultural Research Service
    • Exotic and Emerging Avian Viral Diseases Unit, Southeast Poultry Research LaboratoryUnited States Department of Agriculture, Agricultural Research Service
Original Article

DOI: 10.1007/s00705-010-0813-y

Cite this article as:
Wasilenko, J.L., Arafa, A.M., Selim, A.A. et al. Arch Virol (2011) 156: 37. doi:10.1007/s00705-010-0813-y

Abstract

Domestic ducks have been implicated in the dissemination and evolution of H5N1 highly pathogenic avian influenza (HPAI) viruses. In this study, two H5N1 HPAI viruses belonging to clade 2.2.1 isolated in Egypt in 2007 and 2008 were analyzed for their pathogenicity in domestic Pekin ducks. Both viruses produced clinical signs and mortality, but the 2008 virus was more virulent, inducing early onset of neurological signs and killing all ducks with a mean death time (MDT) of 4.1 days. The 2007 virus killed 3/8 ducks with a MDT of 7 days. Full-genome sequencing and phylogenetic analysis were used to examine differences in the virus genes that might explain the differences observed in pathogenicity. The genomes differed in 49 amino acids, with most of the differences found in the hemagglutinin protein. This increase in pathogenicity in ducks observed with certain H5N1 HPAI viruses has implications for the control of the disease, since vaccinated ducks infected with highly virulent strains shed viruses for longer periods of time, perpetuating the virus in the environment and increasing the possibility of transmission to susceptible birds.

Supplementary material

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Copyright information

© US Government 2010