Archives of Virology

, Volume 155, Issue 6, pp 925–934

Three amino acid changes in PB1-F2 of highly pathogenic H5N1 avian influenza virus affect pathogenicity in mallard ducks

  • Henju Marjuki
  • Christoph Scholtissek
  • John Franks
  • Nicholas J. Negovetich
  • Jerry R. Aldridge
  • Rachelle Salomon
  • David Finkelstein
  • Robert G. Webster
Original Article

DOI: 10.1007/s00705-010-0666-4

Cite this article as:
Marjuki, H., Scholtissek, C., Franks, J. et al. Arch Virol (2010) 155: 925. doi:10.1007/s00705-010-0666-4

Abstract

Despite reports that the PB1-F2 protein contributes to influenza virus pathogenicity in the mouse model, little is known about its significance in avian hosts. In our previous study, the A/Vietnam/1203/04 (H5N1) wild-type virus (wtVN1203) was more lethal to mallard ducks than a reverse genetics (rg)-derived VN1203. In search of potential viral factors responsible for this discrepancy, we found that synonymous mutations (SMs) had been inadvertently introduced into three genes of the rgVN1203 (rgVN1203/SM-3). Of 11 SMs in the PB1 gene, three resided in the PB1-F2 open reading frame, caused amino acid (aa) substitutions in the PB1-F2 protein, and reduced virus lethality in mallard ducks. The wtVN1203 and recombinant viruses with repairs to these three aa’s (rgVN1203/R-PB1-F2) or with repairs to all 11 SMs (rgVN1203/R-PB1) were significantly more pathogenic than rgVN1203/SM-3. In cultured cells, repairing three mutations in PB1-F2 increased viral polymerase activity and expression levels of viral RNA.

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Henju Marjuki
    • 1
  • Christoph Scholtissek
    • 1
  • John Franks
    • 1
  • Nicholas J. Negovetich
    • 1
  • Jerry R. Aldridge
    • 1
  • Rachelle Salomon
    • 1
  • David Finkelstein
    • 2
  • Robert G. Webster
    • 1
  1. 1.Division of Virology, Department of Infectious DiseasesSt. Jude Children’s Research HospitalMemphisUSA
  2. 2.Hartwell Center for Bioinformatics and BiotechnologySt. Jude Children’s Research HospitalMemphisUSA