Archives of Virology

, Volume 155, Issue 5, pp 783–787

Larval stage Lymantria dispar microRNAs differentially expressed in response to parasitization by Glyptapanteles flavicoxis parasitoid

Brief Report

DOI: 10.1007/s00705-010-0616-1

Cite this article as:
Gundersen-Rindal, D.E. & Pedroni, M.J. Arch Virol (2010) 155: 783. doi:10.1007/s00705-010-0616-1

Abstract

MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by targeting messenger RNAs and causing cleavage or translation blockage. miRNAs induced after parasitization of the lepidopteran host Lymantria dispar by the parasitoid wasp Glyptapanteles flavicoxis, which introduces a polydnavirus and other parasitoid factors, were examined to identify induced miRNAs that might regulate host genes and contribute to host immunosuppression and other effects. miRNA profiling of parasitized larval hemocytes versus non-parasitized ones by microarray hybridization to mature insect and virus miRNAs identified 27 differentially expressed miRNAs after parasitization. This was confirmed by real-time relative qPCR for insect miRNAs (dme-mir-1, -8, -14, -184, -276, -277, -279, -289, -let-7) using miRNA-specific TaqMan™ assays. Certain cellular miRNAs were differentially expressed in larval tissues, such as the potentially developmentally linked mir-277, signifying a need for functional studies.

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.US Department of Agriculture, Agricultural Research ServiceInvasive Insect Biocontrol and Behavior LaboratoryBeltsvilleUSA
  2. 2.Department of Veterinary Microbiology and PathologyWashington State UniversityPullmanUSA

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