, Volume 154, Issue 12, pp 1873-1882
Date: 20 Oct 2009

The VP5 protein of infectious bursal disease virus promotes virion release from infected cells and is not involved in cell death

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Abstract

The VP5 protein of infectious bursal disease virus (IBDV) was shown in previous reports to be involved in the cytopathogenicity of IBDV. Here, using a rescued VP5-deficient IBDV infectious clone, it was demonstrated that a lack of VP5 expression significantly hinders the release of viral progeny from infected cells but does not block intracellular virus production. Monoclonal VP5-expressing Vero cells did not exhibit induction of cell death. Using VP5-specific mAbs generated in our laboratory as a tool, it was shown by flow cytometry analysis that VP5 was detectable on the surface of IBDV-infected and monoclonal VP5-expressing Vero cells and bursal cells in IBDV-infected chickens. Taken together, these data suggest that the VP5 protein is involved in regulation of the release of intracellular IBDV virions and may be used as a cell-surface marker for detecting IBDV-infected cells in FCM analysis. This study contributes to the further characterization of the VP5 protein, which will allow a better understanding of the mechanism of IBDV pathogenicity.

Y. Wu and L. Hong contributed equally to this work.