Archives of Virology

, Volume 154, Issue 7, pp 1093–1099

Characterization of SARS-CoV-specific memory T cells from recovered individuals 4 years after infection

  • Yan-Ying Fan
  • Zi-Tong Huang
  • Li Li
  • Man-Hui Wu
  • Tao Yu
  • Richard A. Koup
  • Robert T. Bailer
  • Chang-You Wu
Original Article

DOI: 10.1007/s00705-009-0409-6

Cite this article as:
Fan, YY., Huang, ZT., Li, L. et al. Arch Virol (2009) 154: 1093. doi:10.1007/s00705-009-0409-6
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Abstract

SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular immune response from 21 SARS-recovered individuals who had been diagnosed with SARS in 2003 by using ELISA, CBA, ELISpot and multiparameter flow cytometry assays. Our results demonstrated that low levels of specific memory T cell responses to SARS-CoV S, M, E and N peptides were detected in a proportion of SARS-recovered patients, and IFN-γ was the predominant cytokine produced by T cells after stimulation with peptides. Cytometry analysis indicated that the majority of memory CD8+ T cells produced IFN-γ, whereas memory CD4+ T cells produced IFN-γ, IL-2 or TNF-α. These results might provide valuable information on the cellular immune response in recovered SARS-CoV patients for the rational design of vaccines against SARS-CoV infection.

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yan-Ying Fan
    • 1
  • Zi-Tong Huang
    • 2
  • Li Li
    • 1
  • Man-Hui Wu
    • 2
  • Tao Yu
    • 2
  • Richard A. Koup
    • 3
  • Robert T. Bailer
    • 3
  • Chang-You Wu
    • 1
  1. 1.Key Laboratory of Tropical Disease Control Research of Ministry of Education, Department of Immunology, Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  2. 2.Second Affiliated Hospital of the Sun Yat-Sen UniversityGuangzhouChina
  3. 3.Vaccine Research CenterNIAID/NIHBethesdaUSA

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